The Value Of P27^kip1 And CyclinD₁ In Psoriasis Lesions

Object to investigate the expression of p27^kip1and cyclinD₁ in the skin of patients with psoriasis vulgaris,their correlation and their significance in the development of psoriatic lesions.Methods 48 outpatients with psoriasis vulgaris(who were diagnosed by clinic and pathology and had typical lesions)were studied,including 30 cases in progressive stage and 18 cases in static stage.30 cases of normal skin were chosen as controls.Immunohistochemistry was used to study the expression of p27^kip1(cyclin dependent kinase inhibitor)and cyclinD₁. SPSS 11.5 statistical analysis software was carried out for statistical treatment.Resultsâ‘ p27^kip1protein expression Positive expression of p27^kip1protein was located mainly in nuclear and seldom in cytoplasm.Brown-yellow or brown positive particles were observed.The expression of p27^kip1was significantly lower in lesions with psoriasis vulgaris than in the normal skin controls(P＜0.01).The level of p27^kip1decreased in static lesions in comparison with the normal skin controls(P＜0.05).The level of p27^kip1significantly decreased in progressive lesions in comparison with the normal skin controls(P＜0.01).There was difference in lesional skin between progressive stage and static stage(P＜0.01).â‘¡cyelinD₁ protein expression Positive expression of cyclinD₁ protein was located in nuclear. Brown-yellow or brown positive particles were observed.There was significant increase of cyclinD₁ in lesions with psoriasis vulgaris in comparison with those of the normal skin controls (P＜0.01).The level of cyclinD₁ increased in static lesions in comparison with the normal skin controls(P＜0.01).The level of cyclinD₁ increased significantly in progressive lesions in comparison with the normal skin controls(P＜0.01).And the expression of cyclinD₁ was higher in lesions of progressive stage than of static stage(P＜0.05).â‘¢There was negative correlation between p27^kip1and cyclinD₁ in psoriasis vulgaris(r=-0.302).Conclusionâ‘ The down-regulation of p27^kip1is likely to relate to overproliferation of keratinocytes in psoriatic lesions.â‘¡The expression of cyclinD₁ was significantly higher in psoriatic lesions than that in the controls.It was also significantly higher in progressive lesions than that in static lesions.CyclinD₁ is a factor in evaluating hyperplasia of keratinocytes in psoriasis vulgaris.â‘¢In the progress of psoriasis vulgaris,they regulated each other.