Effects Of Propofol On Hepatic Cell Apoptosis Induced By Intestinal Ischemia-Reperfusion Injury And Expression Of Caspase-3 In Rats Liver

Objective To investigate the effects of propofol on hepatic cell apoptosis and expression of Caspase-3 with intestinal ischemia-reperfusion injury in rats.Methods Fifty-four healthy Wistar rats weighing 200-250g were randomly divided into 3 groups as follows(n=18):compared group(C group),ischemia-reperfusion group(IR group),propofol group(P group)and midazolam group(M group).The rats were executed in each group after reperfusion for 1h,3h and 6h respectively.A rat model of intestinal ischemia-reperfusion was established by blocking the superior mesenteric artery with clap.The left hepatic lobes were sampled at the end of reperfusion.Light microscopy and electron microscopy were performed to verify the morphologic and ultrastructural changes of hepatic cell apoptosis.Caspase-3 protein expression in liver tissues was assessed by immunohistochemical method. The apoptosis positive numberof hepatic cell was measured by TUNEL method.Resultsâ‘ The result of light microscopy and electron microscopy:Compared with C group,IR group showed great changes.The pathological lesions were lighter in P group than in IR group.â‘¡The result of Apoptosis positive numberof hepatic cell:The apoptosis positive numberof hepatic cell were significantly higher in IR,P groups than in C group(P<0.05).AI was significantly lower in C,P groups than in IR group(P<0.05).The result in two time phases of group IR,P reached the peak at 6h after reperfusion(P<0.05).â‘¢The expression of Caspase-3:Caspase-3 protein expression were significantly higher in IR,P groups than in C group(P<0.05).Caspase-3 protein expression was significantly lower in C,P groups than in IR group(P<0.05).Expression of Caspase-3 protein had not on significant difference between 3 time points in 3 groups(P>0.05).Conclusionsâ‘ Hepatic cell apoptosis participate in the injury induced by intestinal ischemia reperfusion.â‘¡Propofol can relieve the liver cellular apoptosis by decreasing Caspase-3 protein expression.It may protect liver from intestinal ischemia-reperfusion injury.