Experimental Investigation Of The Reversal Effect Of SC On Human Multidrug Resistance Breast Cancer Cell Line

Objective:To explore the reversal effect of stellera chamaejasme L.crude extract(SC) on SGC7901/ADM gastric cancer cell line.Methods:The inhibition rate of different concentration of SC to SGC7901/ADM cells were evaluated with MTT assay.The inhibition rate of adriamycin(ADM) to SGC7901/S,SGC7901/ADM and SGC7901/ADM plus SC were evaluated with MTT assay.The IC₅₀ of different agents was counted.The SGC7901/ADM cells intracellular concentration of ADM and the expression of P-gp with and without SC was detected by flow cytometer (FCM).To analyse the express of caspase3 in SGC7901/ADMinterfered by SC.Results:SC at the concentration of 0.25 mg/ml had no significant cytoxicity to SGC7901/ADM cells.When 0.25 mg/ml SC was added along with ADM toSGC7901/ADM culture in vitro,the medium inhibited concentration(IC₅₀) was evidently reduced and ADM resistance was partly overcome.The reversal fold was 2.54,0.25 mg/ml SC could significantly increase the intracellular accumulation of ADM and decrease the expression of P-gp in SGC7901/ADM cells(P＜0.05);The positive grains of caspase3 primarily located cytoplasm near the nucleumenbrane.he express of caspase3 in SGC7901/ADM cell line was little without the interference of SC,the brown grains were little and the color was light.The brown grains gradually increased namely the express of caspase3 increases with the increase of gradually increased namely the eapress of caspase3 increases with the increase of dose and time.Conclusion:SC had reversal effect on the multidrug resistance in SGC7901/ADM cells. The reversal mechanism might be related to the down-regulation of the expression of P-gp.The function of renersing MDR may be related to caspase3 protein family of apoptosis roads...