Secondary Metabolites Of Stellera Chamaejasme The Ground Study On The Biological Activity And Chemical Characteristics

The paper's study object is above part of Stellera chamaejasme L., especially its leaves. The study went on three aspects: antitumor and antibacterial activity, components of bioactive substances and their diversity and dynamic distribution.The study result on the antitumor activity indicated: three different polar extracts of Stellera chamaejasme L.'s leaves-A, B and C had obvious antitumor activity. Their activities of inhibition on S-180 were 42.689%, 42.559% and 36.771%, separately.The study on antibacterial activity showed the compound 3 inhibited the Escherichia coli obviously when the concentration is 1/10000. At the same time, the inhibition activity on Staphylococcus aureaus is stronger than that on Escherichia coli when the concentrations were same. Study on the secondary metabolites' bioactive activity provided basis for the exploitation of above part of Stellera chamaejasme L.Through solvent-extraction, silica gel TLC and UV, 'H-NMR, 13C-NMR, MS, est., daphnetin and daphnin were separated and identified from Stellera chamaejasme L.'s leaves. They all had antitumor activity and others.Five compounds were separated and identified by GC-MS from Stellera chamaejasme L.'s leaves. The result showed there were 26 components found in the plant for the first time. They were: 2-hydroxy-methyl Propanoic acid, 4-Methyl-1-hepten-4-1, 5-Methyl-3-Hexanol, 1-Cyclopentyl-1-propanol, 2,6,11-trimethyl-Dodecane, 2-Butyl-1-Octanol, 1-Octadecyne, 3,7,11,15-Tetramethyl-2-hexadecen-1-l, 2,4-dimethyl-3-Pentenal, borneel, 2-propyl-l-Heptanal, Heptacosane, Eicosane, Heneicosane, Docosane, Pentacosane, Octacosane, Nonadosane, Pentatriacontane, 2,6,10,14-tetramethyl-2-Hexadecene, Nonadecanol, oleic acid, 1-Tetracosanol, 9,12-Octadecadienoic acid methyl ester, Octadecatrinoicacid, Benzothiazole.The means of HPLC, which identified the content of daphnetin, was studied carefully for its methodology. The condition of HP-1050 determining thedaphnetin was: chromatogram colum: Allteck C18(250mm +4.6mm, 5um); mobile phase: methanol : water-40:60; wavelength: 310nm; temperature: 28 C; injection quantity: 10ul. The sample's extraction condition: weighed l.000g crushed leaves of Stellerachamaejasme L. exactly, then extracted it by ultrasound in 5ml 75% ethanol solution for 1.5 hour, filtrated and kept its volume in 10ml volumetric flask.During the spectral purity identification on sample and standard sample, the check result of spectral purity factor was 100%. The result tested that the purity of identified compounds was normative, the method of pre-management was correct. So the conclusion about data was creditable.The distribution of daphnetin's content in above part of Stellera chamaejasme L. was: 1.76% in leaves, 1.60% in flowers, 0.58% in stems, and 0.37% in roots. So we can conclude that daphnetin's content was highest in the leaves, and the content in above part of plant was more than that underground, daphnetin's dynamic distribution and diversity provided exploitation feasibility on the above part of Stellera chamaejasme L.Take leaves as example, daphnetin's content was discrepant in different areas: 1.76% in Daode zone, 0.73% in Kerqin, and 0.81% in Changling. It indicated there existed difference on the Stellera chamaejasme L.'s medicine value in different areas. As far as daphnetin, Stellera chamaejasme L.'s medicine value coming from Daode was better than other areas.