The Study Of The Expression Of TSLC1 In Laryngeal Squamous Cell Cacinoma

Objective To investigate the correlation of TSLC1 and laryngeal squamous cell carcinoma by detecting the difference of TSLC1 expression in laryngeal squamous cell carcinoma and laryngeal carcinoma adjacent normal tissue to provide a theoretic basis of oncogenesis,diagnosis and treatment.Methods 45 paraffin-embedded laryngeal squamous cell carcinoma specimens were selected to be used to investigate.The average age of the patients of laryngeal squamous cell carcinoma was 61.2 years,of whom,there are 43 males and 2 females,and there are 8 patients with metastasis of lymph nodes.20 laryngeal carcinoma adjacent normal tissue were selected to be control.The expression of TSLC1 were detected by Immunohistochemical staining(PV-6000).The relations of the expression of TSLC1 in laryngeal squamous cell carcinoma specimens and the clinicopathological parameters of the patients were analyzed by SPSS10.0.Results The positive expression of TSLC1 was 17/45(37.8%)in specimens of laryngeal squamous cell cacinoma,which was significant lower than in specimens of laryngeal carcinoma adjacent normal tissue(75%),the difference was significant(X~2=7.68,P<0.01),and loss of TSLC1 expression was correlated with the clinical stage,the tumor differentiation grade and the lymph node metastasis(X~2=4.15,6.11,4.11 P<0.05),but not correlated with the age,location of primary Laryngeal carcinoma and gender(X~2=0.25,0.09,1.0 P>0.05).Conclusion Loss of TSLC1 expression was frequently observed in laryngeal squamous cell cacinoma,and loss of its expression was significantly correlated to the clinical stage,the tumor differentiation grade and the lymph node metastasis,suggesting loss of TSLC1 may contributed to the occurrence and invasion of laryngeal squamous cell cacinoma.Further investigations are needed to elucidate the mechanism by which TSLC1 is downregulated and how loss of TSLC1 influences proliferative activity and invasiveness of laryngeal squamous cell cacinoma.