Preliminary Study On Chemical Components And Antitumor Activity Of The Stem Of Angelica Dahurica

Angelica dahurica(Fisch.) Benth.et Hook,a perennial herb in genus angelica L.of family Umbelliferae,which chiefly distributes in Heilongjiang,Jilin,Liaoning,Neimenggu, Shanxi,Hebei province of China,is a traditional chinese medicine.The study carries out the chemical constituents and the pharmacology effect of Angelica dahurica.1.A method for the determination of imperator in in Angelica dahurica by HPLC was established.The HPLC separation was proformed on a hypersil ODS2 column (250mm×4.6mm,5μm),at flow rate 1mL/min,with mobile phase of methanol/water(65/35, V/V) and detected at wavelength 254 nm.Using Uniform design,the optimal conditions for imperatorin extraction were explored to be ultrasonically extracted by 78%ethanol at 20℃for 22 min.Imperatorin contents in Angelica dahurica from different area were determined.The results showed that imperatorin contents in Angelica dahurica from different area were remarkably different,to the stem,the highest imperatorin content was 0.018%,from Tahe.So, the Angelica dahurica stem from Tahe was used in following experiments.2.The essential oil from the stem of Angelica dahurica was extracted by steam distillation method.The oil was analyzed by GC-MS,79 components were identified,The major constituents are fatty acid ester,acid and sesquiterpenoids.Among the 79 constituents,74 of them are not found in the root of Angelica dahurica,except other five constituents which are 1-(1,5-dimethyl-4-hexenyl)-4-methyl-benzene,3-methyl-butanoic acid hexadecyl easter, hexadecanoic acid ethyl ester,methyl linolenat,and ethyl oleate.Analyzing the chemical constituents of essential oil from the stem of Angelica dahurica can provide scientific foundation for further development and utilization of it.3.The chemical constituents of the stem of Angelica dahurica(Fisch.)Benth.et Hook is studied,.At frist,extrat the coarse powder of Angelica dahurica with 80%ethanol,secondly, extrat the ethanol extraction with ethyl ether,ethyl acetate in turn,then We get three part,the part of ethyl ether,ethyl acetate,and water.Then separate and purified the three part respectively using the silica gel column chromatography and thin-layer chromatography. Finally,10 compounds have been separated from the plant the stem of Angelica dahurica.4.The influence of purified compounds and crude exstracts from the stem of A.dahurica on tumor cells' activity were determined,and seven cell lines were used in the experiment, HT29 Colon cancer cell line,DU145 Prostate Carcinoma cell line,Mia PaCa-2 Human Pancreatic carcinoma cell line,T47D Breast Carcinoma cell line,Brown melanoma cell line, 2774 ovarian carcinoma cell line,and Spark Lung carcinoma cell line.the results showed that the anti-tumor activity of compound 5 was obviously.