| Objective: Up to now the cholecystectomy is still the head curing method for the gall bladder pathological changes. Recent years, the improvement of medical equipment along with diagnoses technology is raised, as well as laparoscopic cholecystectomy made a great satisfactory in the area of Minimally Invasive Surgery there has been a large increase in annual cholecystectomy rates in our country. There are many pathophysiology changes and complications after cholecystect- omy. Stool habit and stool trait are changed in some people. And even patients have persistence of one or two symptom such as epigastrium abdominal pain, abdominal distension, diarrhea and nauseates and detests greasy etc, is called" Postcholecystectolmy Syndrome (PCS)", which makes patients unconvenient in life and work. Diarrhoea which has different duration is the most common symptom; however, serious symptoms like abdominal pain and critical diarrhoea seldom appear. Stool has great different traits among different patients and antibiotic can not relieve the symptom. The research about its cause is seldom published domestic and abroad. Therefore the diarrhoea after cholecystectomy must be paid much more attention to. The intention of this trial is tring to finding out whether or not influencing the intestines movement, by way of testing the change of bile acid, bilirubin, cholesterol in the blood, bile, jejunum, ileum, colon and intestinum rectum after cholecystectomy, interprete the relations between the malabso- ption of bile acid and diarrhea after cholecystectomy, in order to provide the theoretical basis about the prevention and cure the patients'diarrhea after cholecystectomy.Method: 80 clean healthy suckling pigs, weighing 250-300g, are ramdomly divided into 4 groups: normal control group A, cholecystectomy group B, normal control group C, cholecystectomy group D, with 20 suckling pigs in each group. The animals in group A and C were performed sham operation, remaining the cholecyst. The animals in group B and D are given operations of cholecystectomy. All of the operations are sterile. Observe the conditions of the animals'stool and diet, when the animals recover normally, about 3 weeks later, fast 12h,don't drink 4h, in the group A and C assemble the blood samples from the heart, collect 1ml bile through one tube which we insert into the common bile duct, The collection of specimen: 1ml gall from the choledoch intubation, the content in jejunum that is 25cm from 15cm down the top to the remote, content in ileum that is 25cm from remote to the proximal end, content in colon that is 20cm from the top to the remote, and 6 night soil balls up from the terminal of recta. Detect the concentration of the bile acid, bilirubin and cholesterol in the blood, bile, intestinal juice of jejuna and ileum, oven dry the stool of the colon and intestinum, weighing 200mg, grind it down, misce bene with 2ml distilled water thoroughly, then detect the concentration of the acid bile, bilirubin, cholesterol.In the group C and D, anest- hetize through abdominal cavity. Open anmials abdominal, pures Barium Sulfate emulsion into cavity 5cm ahead of pylor- us and cecum's root. Then put the bowel back and closes down the abdominal cavity.30 minutes later, make animals death, cut out the stomach and bowel, take the full intestines hanging down with 5 gs' scales weights.Pylorus and ileocecal valve are the two starting points, measure migration length of each group in small intestine and colon.Gets the whole length of small intestine (from pylorus to ileocecal valve) and colon(from ileocecal valve to anus). Calculates the migration percentage (distance Barium Sulfate moved/ the whole length of small intestine or colon) of each group.The experimental date was demonstrated in mean±standard (x±s).The analysis of variance and t test were used to the significance test by SPSS10.0 statistic software.Results:1 group A and group B:compared the concentration of bile acid in the blood: the concentration of bile acid in group A is 13.80±3.23μmol/L, the group B is 11.3±3.43μmol/L, there is significant difference.2 group A and group B:compared the concentration of bile acid in the bile: the concentration of bile acid in group A is 2480.23±23.52μmol/L, the group B is 2439.13±23.79μmol/L, there is significant difference.3 group A and group B:compared the concentration of bile acid in the jejunum: the concentration of bile acid in group A is 2280.65±22.78μmol/L, the group B is 2230.23±21.76μmol/L, there is significant difference.4 group A and group B:compared the concentration of bile acid in the ileum: the concentration of bile acid in group A is 52.32±6.43μmol/L, the group B is 47.34±5.87μ mol/L, there is significant difference.5 group A and group B:compared the concentration of bile acid in the colon: the concentration of bile acid in group A is 20.20±5.53μmol/g, the group B is40.40±4.97μmol/g, there is significant difference.6 group A and group B:compared the concentration of bile acid in the intestinum rectum: the concentration of bile acid in group A is 8.91±4.89μmol/g, the group B is 18.12±4.43μmol/g, there is statistical significance.7 group A and group B:compared the concentration of bilirubin in the blood: the concentration of bilirubin in group A is 0.05±0.55μmol/L,the group B is 0.06±0.51μmol/L,there is no significant difference.8 group A and group B:compared the concentration of bilirubin in the bile: the concentration of bilirubin in group A is 27.06±4.21umol/L, the group B is 17.9±3.98umol/L, there is significant difference.9 group A and group B:compared the concentration of bilirubin in the jejunum: the concentration of bilirubin in the jejunum in group A is 16.92±4.11μmol/L, the group B is 11.52±3.89μmol/L, there is significant difference.10 group A and group B:compared the concentration of bilirubin in the ileum: the concentration of bilirubin in group A is 2.61±3.67μmol/L, the group B is 0.91±3.78μmol/L, there is significant difference.11 group A and group B:compared the concentration of bilirubin in the colon: the concentration of bilirubin in group A is 3.81±4.65μmol/g, the group B is 4.20±3.65μmol/g, there is significant difference. 12 group A and group B:compared the concentration of bilirubin in the intestinum rectum: the concentration of bilirubin in group A is 1.81±4.43μmol/g, the group B is 2.10±3.56μmol/g, there is significant difference.13 group A and group B:compared the concentration of cholesterol in the blood: the concentration of cholesterol in group A is 1.83±4.56mmol/L, the group B is 1.62±3.76mmol/L, there is significant difference.14 group A and group B:compared the concentration of cholesterol in the bile: the concentration of cholesterol in group A is 0.01±0.11mmol/L, the group B is 0.12±0.17mmol/L, there is no significant difference.15 group A and group B:compared the concentration of cholesterol in the jejunum: the concentration of cholesterol in group A is 0.23±0.15mmol/L, the group B is 0.25±0.13mmol/L, there is no significant difference.16 group A and group B:compared the concentration of cholesterol in the ileum: the concentration of cholesterol in group A is 0.006±0.07mmol/L, the group B is 0.005±0.08mmol/L , there is no significant difference.17 group A and group B:compared the concentration of cholesterol in the colon: the concentration of cholesterol in group A is 0.005±0.08mmol/g, the group B is 0.006±0.07mmol/g, there is no significant difference.18 group A and group B:compared the concentration of cholesterol in the intestinum rectum. the concentration of cholesterol in group A is 0.01±0.09mmol/g, the group B is 0.01±0.07mmol/g, there is no significant difference19 group C and group D:Compared the migration percentage of small intestine: group C (40.41±3.26%), groupD (56.91±3.24%), the value in group C and group D different (P<0.05).20 group C and group D:Compared the migration percentage of colon: group C (6.64±4.5%), groupD (9.26±3.26%), the value in group C and group D different (P<0.05).Conclusion: After postcholecystectomy, small intestine or colon peristalsis function were strengthened, the concentration of acid bile in the blood serum, bile, jejunum and ileum degrades; the concentration of colon and intestinum rectum increases; it can be the cause of diarrhoea after cholecystectomy.
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