| Objective:In recent years many investigations are focus on the immunological factors of infertility and investigators pay much attention to antisperm antibody(AsAb) and anticardiolipin antibody(ACA) among those factors.Antisperm antibody is a hidden antigen for men and is a foreign antigen for women,but normally there is no AsAb ,because of some physiological protective mechanisms.If these physiological protective mechanisms are destroied AsAb will be produced.ACA is one of anti-phospholipid antibodies,it has negative charge ,normally ACA locates in the inner layer of double-layer lipid cellular membrane and is not be detected by immune system .If cuorin is exposed ACA will be produced.This study is designed to detecte the expression of three subtypes (IgG,IgM,IgA ) of AsAb and ACA and to analyze the function of AsAb and ACA in infertility.Method: 1 Object:50 infertile couples were choosed from out-patient department(OPD) of urology of the thrid hospital of He Bei Medical University and OPD of andrology of Bai Qiuen international peace hospital during may 2008 to october 2008 as experimental group(the age of men 25～31,mean 26.7士1.67;the age of women 23～32,mean 26.22士3.05).All of these couples did not detect AsAb and ACA before.Election qualification: They married over one year,have normal sexual life and do not use any contraception but they are infertile .All the men do not have genetic disease family history,have no chronic disease and have normal testicle,epididymis and deferent canal. All the women do not have genetic disease family history,have no chronic disease and follicular development are normal,they have normal ovulatory cycle,vagina,uterus,ovarian duct and ovaries.Normal control group:40 healthy couples(the age of men 24~31,mean 26.4士1.85;the age of women 23~33,mean 26.63士3.47 ) who have normal childbearing history but are not pregnant when they are detected,the urinarysurgery and gynecologic examinations are normal.2 Sample collection: Taking 3ml venous blood in the morning but donot anticoagulate, abstracting serum and then put the serum in -20℃refrigerator.Collecting and analyzing semen after sexual abstinence for 5-7days.3 Detection Method: Choosing ELISA to detect AsAb and ACA in serum.we buy ELISA kit form Shenzhen Anqun Biotechnology Company limited and follow the kit instruction when doing experiment and assessing results.4 Statistic Analysis: The result of AsAb and ACA are represented by numeration data and the Data are analyzed through x2 or fisher's exact test. The results of semen parameter are represented by x±s and the Data are analyzed through tort′test. All the data are analyzed in computerized SAS8.1software. For each of these analyses, two-tailed probability values <0.05 are designed as significant.Result: 1 The result of AsAb and ACA:The positive rate of AsAb in male infertile group is higher than control group and the difference is significant(30 % vs 5%,P<0.01)see Fig1 andTable3. The positive rate of AsAb in female infertile group is higher than control group and the difference is significant(34% vs 12.5%,P<0.05)see Fig1 and Table5.The difference of positive rate of ACA between infertile male group and control group is no significant(6% vs 5%,P>0.05)see Fig1 and Table4. The positive rate of ACA in female infertile group is higher than control group and the difference is significant(32% vs 10%,P<0.05)see Fig1 and Table6.2.Semen analysis:When comparing semen parameter between AsAb positive but ACA negative infertile men in the experimental group and AsAb negative and ACA negative fertile men in the control group,there is no difference in capacity of semen,PH,density of sperm and sperm in B stag(eP>0.05).But the motility rate of sperm,sperm in A stage,rectilinearity,linear velocity and curve velocity in control group are hingher than experimental group and the differences are significant(P<0.01) see Table7.Conclusion: 1 AsAb and ACA are imporant immunological factors that can cause infertility, AsAb can damage male and female potentia generandi but ACA only decrease female potentia generandi .2 AsAb can impact the quality of semen and then result in infertility.
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