Experimental Study Of The Expression Of Cyclin G1 And NGF In The Process Of Tissue Engineering Neural Graft Repair Of Rat Sciatic Nerve Defect

Peripheral nerve defects caused by trauma are common during clinical practice,to recover the nerve function we must recover the nerve continuity.Tissue engineering which was emerged in the 80s of 20^th century provides a new research direction for the recover of peripheral nerve defect.Acellular nerve allograft stent after the chemical methods treatment is a new type of tissue,engineering material.The study showed, planted bone marrow-derived mesenchymal stem cells(BMSc),which promoted nerve growth,in the artificial neural stent bridging nerve defects,there was good for nerve regeneration promotion role.The mechanism of nervous system regeneration after injury is complex.Nerve growth factor(NGF) can promote neuronal survival and regeneration.Cyclin G1 is a new cyclin,and its function has not yet been fully understood clearly,but the study shows that Cyclin G family must play a role in the cell growth process.Morila etc, found that after nerve injury,the expression levels of Cyclin G1 in the single nucleus neurons cells significantly increased at neural repair process,which indicated that Cyclin G1 may play a role in nerve cell regeneration process.At present,there are very few literature reported Cyclin G1,may be associated with nerve injury regeneration.In this experiment,we draw assistance from relevant animal experimental model,utilize SP immunohistochemical method to observe protein level of Cyclin G1 and NGF in the process of artificial neural bridge repair,to explore the potential relevance of Cyclin G1 protein expression in artificial neural bridge repair, and provides an experimental basis for the next step study.Materials and MethodsSpecimenswe use animal experiments specimens of tissue-engineered artiticial neural repair of peripheral nerve injury.WaysImmunohistochemistry(S-P) reactionTreatment of the resultsimage analysis,statistical software SPSS10.0ResultsImmunohistochemical staining results shows that the positive protein expression part of cyclinG1 and NGF was located in the nucleus.The average integrated of cyclinG1_in the process of peripheral nerve defect repair by tissue-engineering neural graft,tissue-engineering artificial nerve and.autologous nerve was respectively 32.7072;15.1750;12.8776.The average integrated of NGF in the process of peripheral nerve defect repair by tissue-engineering neural graft,tissue-engineering artificial nerve and_autologous nerve was respectively 51.0900;15.7400;14.7110. Statistical results suggest that the expression differences between cyclinG1 and NGF in the process of peripheral nerve defect repair by neural graft,autologous nerve,and artificial nerve are all have statistical significance(p＜0.05),it shows that cyclinG1 and NGF protein increased the expression in the process of tissue-engineered nerve stent repair of peripheral nerve defect.Conclusion1.During the neural tissue engineering scaffold process of repair of peripheral nerve defect in a nerve injury of the regeneration phase,protein expression of cyclinG1 increase.It shows that the CyclinG1 may be associated with nerve regeneration and development mechanism.2.During the neural tissue engineering scaffold process of repair of peripheral nerve defect in a nerve injury of the regeneration phase,protein expression of NGF increase. It shows that the NGF may be associated with nerve regeneration and development mechanism.