| ObjectiveIn cadiac surgery,myocardial ischemic reperfusion injury(MIRI) is a frequent tissue and organ injury,following the development of organ transplantation and other complex surgery,people pay more attention to MIRI.But up to now,The pathogenesis is not completely elucidated,and it is commonly thought that this process is related to oxygen free radical,calcium overload,apoptosis and so on.It is reportet that heat shock protein 70(HSP70)can protect myocardial contractile force,reduce the area of heart muscle ischemia and antiarrhythmia,by induce the production of HSP70 can ease MIRI. For the past few years,the prevention of MIRI have already acquired much progression by using of the traditional Chinese medicine.Xue Bi Jing Injection is extracted from 36 prescriptions of traditional Chinese medicine,the active ingredient are Danshen root, Chuanxiongzine,ferulaic acid,peoniflorin,safflor yellow A etc,having the properties of anti-bacterial toxin,decreasing the level of endotoxin,regulating immunity and mediators of inflammation,improving microcirculation,protecting vascular endothelial cells etc.Also it can protect and repair organs and system by improving the supeoxide dismutase activity.The main task of this research is to observe the protection of Xue Bi Jing Injection to MIRI and to approach the relationship of heat shock protein 70 expression by using of the isolated Langendorff perfusion model,so that we can make good use of it. 2.model building:10%Chloral Hydrate intraperitoneal injection anesthesia induction,then heparin-sodium intraperitoneal injection,hearts were excised rapidly,in chilled 4℃Krebs-Henseleit buffer(K-H buffer),connecting the aortic cannulation to the the isolated Langendorff perfusion model,and then perfused,at a constant perfusion pressure of 75 cmH2O,with 37℃oxygenated K-H buffer.After 15 minutes equilibration,control group were perfused with St Thomas II solution,and received 30 minutes of global ischemia,then reperfused with K-H solution at 37℃until 120 minutes;test group added Xue Bi Jing Injection in St ThomasⅡsolution,other procedures were the same to control group.The samples of myocardium of left ventricle were collected after the experiment is completed,then be fixed in 10% formaldehyde and preserved at 4℃refrigerator.Other tissue was frozen rapidly and stored at -70℃refrigerator.3.collection and samples:We collected and measured the cornory flow from equilibrate perfusion 10 to 15 minutes and 25 to 30,85 to 90 minutes of reperfusion respectively,took 3ml preserved at -20℃refrigerator for CK detecting using automatic biochemistries' detecting instrument,and detected heat shock protein70 in the tissue which stored at -70℃refrigerator with ELISA.Then viewed the morphology changes of myocardium by optical microscope.4.Statistical analysis:All statistical processes were completed via SPSS(V13.0).All measurement data were expressed as mean±standard error(x±s),and statistical significance was evaluated by multiple comparison of t test and ANOVA.Differences were considered to be statistically significant when P≤0.05Results1.Compared with control group,the expression of heat shock protein 70 was significantly increased in test group after reperfusion(P＜0.05).2.Compared with control group,the expression of Creatin kinase at pre-ischemia were not significant(P＞0.05);However,the decreases of Creatin kinase were significant in test group at 30 and 90 minutes after reperfusion(P＜0.05).3.Compared with control group,Coronary flow was not significant at pre-ischemia(P＞0.05),but it was significant raised in test group at 30 and 90 minutes after reperfusion(P＜0.05).4.Myocardial morphology changes:in test group,the cadiocyte are normal on the whole and inflammatory cell infiltration is not very obviously,compared with endocardium inflammatory cell infiltration and aggregation in control group.Conclusion1.Xue Bi Jing Injection can significantly increase the expression of heat shock protein 70 in myocardium after reperfusion and maybe provide myocardial protection during MIRI by this way.2.Xue Bi Jing Injection could protect myocardium by increase Coronary flow, decrease the release of Creatin kinase.3.Xue Bi Jing Injection could maintain myocardial cell,release inflammatory reaction so that make protection of cardiac muscle cell.
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