Effects Of Reinforcing Qi And Warming Yang Prescription On Calcium Overload Of The Myocardial Ischemia-Reperfusion Arrhythmia Model Of Rats

Objective:By setting up the myocardial ischemia-reperfusion arrhythmia model of rat and producing relative experimental evidences,we would probe the mechanism and theory of reinforcing qi and warming yang prescription protecting myocardial ischemia-reperfusion arrhythmia,and provide an experimental basis for further investigating and clinical use.Method:40 healthy wister rats were divided into four groups at random: the group of pseudo operation;the group of ischemia-reperfusion operation; the group of traditional Chinese medicine;the group of propafenone.The model of myocardial ischemia-reperfusion injury was created with the ligation of left anterior descending coronary artery lasting 10minutes and postischemiac reperfusion persisted 30minutes also.We observed the incidence of reperfusion arrhythmias,the activities of Na⁺-K⁺-ATPase,and Ca²⁺-Mg²⁺-ATPase in myocardial tissues and the calciumion content of myocardial tissue.Result:l.The incidence of reperfusion arrhythmia of the group of traditional Chinese medicine was much lower than the group of ischemia -reperfusion operation(P＜0.01),but not significantly altered with the group of propafenone(p＞0.05).2.Compared with the group of pseudo operation,the activities of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in myocardial tissues of the other groups were much lower(P＜0.01).The activities of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in myocardial tissues of the group of traditional Chinese medicine were much higher than the group of ischemia-reperfusion operation(P＜0.01),but not significantly altered with the group of propafenone(p＞0.05).3.Compared with the group of pseudo operation,the content of Ca²⁺ in myocardial tissues of the other groups were much higher(p＜0.01).The content of Ca²⁺ in myocardial tissues of the group of traditional Chinese medicine was lower than the group of ischemia-reperfusion operation(P＜0.01),but not significantly altered with the group of propafenone(p＞0.05).Conclusion:Reinforcing qi and warming yang prescription has protective effects on the rats with myocardial ischemia-reperfusion arrhythmia.The mechanism of action is related to decreasing the calcium overload and increasing the ATPase activity.