The Anti-angiogenic Effect Of Xiaoaiping On Lewis Lung Carcinoma In C57BL/6 Mice

ObjectsXiaoaiping(XAP) isolated from the stems of Marsdenia tenacissima.The traditional Chinese medicine realized that Marsdenia tenacissima has a lot of effects in clearing away the heal,expelling superficial evils,decreaing inflamation and pain, relieving cough and asthma.The studying of pharmacology for Marsdenia tenacissima has found that steroid glycoside and polysaccharrides which were isolated from Marsdenia tenacissima,have an obvious effects against cancer recently.XAP may promote cell differentiation,induce apoptosis of cancer cells in vitro study and inhibit tumor growth in vivo experiment.Using of XAP lonely or combined with chemotherapy,radiotherapy has been confirmed a thrilling treatment of effectiveness on patients in clinical practice,including esophageal cancer,gastric carcinoma, hepatic cancer,lung cancer,malignant dropsy of serous cavity and so on. Unfortunately,the specific mechanism of curing cancer were not clear.The experiment intents to investigate the influence of XAP in Lewis lung carcinoma in C57BL/6 mice and to clarify the specific mechanism of anti-tumor.Methods1.Forty-five C57BL/6 female mice which injected Lewis lung carcinoma cell were randomly divided into three groups,that was group A,B,C,and with fifteen mice every group.Group A was peritoneal injected with cisplatin(DDP),Group B was peritoneal injected with XAP and Group C was peritoneal injected with NS.2.After three week's treatment,the mice were sacrificed and taking blood from eyeballs,and the tissue of xenografted tumor was taken and weighed.3.The level of CD₃₄ in xenografted tumor and the expression of microvessel density (MVD) were detected by imrnunohistochemical image analysis.The concentration of serum VEGF,bFGF were measured by ELISA.4.Thirty C57BL/6 female mice which injected Lewis lung carcinoma cell were randomly divided into two group.Group A was peritoneal injected with XAP and Group B was peritoneal injected with NS.The days of survival time of mice were recorded after three week's treatment.Results1.Compared with Group NS,there was significantly difference in xenografted tumor.The inhibition rate was 15.69%.2.Compared with Group NS,there were significantly difference in the indexes such as MVD,concentration of serum VEGF and bFGF.3.Compared with Group NS,there was significantly difference in survival time. The extended rate was 19.16%.Conclusions 1.XAP can inhibit tumor growth of Lewis lung carcinoma in C57BL/6 mice.2.XAP can prolong the survival time of C57BL/6 mice with Lewis lung carcinoma.3.XAP may down-regulate VEGF,bFGF expression and decrease MVD to inhibit tumor angiogenesis of C57BL/6 mice with Lewis lung carcinoma...