Study On The Correlation Between Serum TPOAb And Expression Of Sexual Hormone Receptors And TPO In Endometrium With DUB

ObjectiveDysfunctional uterine bleeding(DUB) is an common disease for female,but the exact mechanisms behind the bleeding are uncertain.It is always think that the main cause is dysfunction of the neuroendocrine.Recently,it is found the micro-Environment play a important role in DUB.We found some of the DUB patients have the antithyroid antibodies(ATA) positive,but FT3,FT4 and TSH are normal in clinic,so we doubt is there a related link between DUB and sub-clinical AITD? The purpose of this study was to investigate the expression of TPO in human uterine endometrium,combine with the examination of the serum,to explore the relationship between dysfunctional uterine bleeding and AITD,and provide a new way for DUB'treatment.MethodsImmunohistochemistry was used to exam the expression of ER,PR,AR in endometrium of 34 DUBs and the expression of TPO in all the cases of our study which were involved in 2007.12～2008.11.Mean while,we chose Chemiluminescence to exam the levels of FT3,FT4, TSH and TPOAb in serum of all the cases.Results1.There are 9 DUB patients have serum TPOAb positive,the positive rate is 26.5%; but only 1 of the controls has serum TPOAb positive,and the rate is 5%.It means the level of TPOAb in DUB is much higher than the controls.2.There is no significant difference in the expression of ER,PR and AR between the patients of serum TPOAb positive and negative.3.The results of Immunohistochemistry suggested that TPO exists in endometrium, and the positive expression was in plasma of all kinds of the endometrial cells.We found all the samples which expressed positive results in serum TPOAb have weaker expression in Immunohistochemistry,and there is a negative correlation between serum TPOAb and the expression of TPO in endometrium,R=-0.571 Conclusions1.Considerable DUB patients have serum TPOAb positive,it suggests that these patient perhaps have sub-clinic AITD.2.The levels of serum TPOAb in DUB patients is not relevant with the expression of ER,PR or AR in their endometrium.3.TPO expresss in women's endometrium,it exists in plasma of all kinds of the endometrial cells.There is a negative correlation between serum TPOAb and the expression of TPO in endometrium.