Effect Of The Oxcarbazepine On Bax And Bcl-2 Expression Of Hippocampus Epilepsy Of Rats Chronically Kindled By PTZ

Objective:To studay the effects of the Oxcarbazepine on Bax and Bcl-2 expression of hippocampus in seizures rats induced by PTZ,and to explore the protection of the different dosage of Oxcarbazepine for the neurons of the hippocampus.Methodes:Eighty adult Wistar rats were randomlydivided into normalcontrol (NP)group,PTZ group,Valproate Sodium(VAP) durg positive Low dose group(25 mg/kg /d),medium dose group(38 mg/kg/d),high dose group(76 mg/kg/d), Oxcarbazepine(OXC) treatment Low dose group(165 mg/kg/d),medium dose group(250 mg/kg/d),high dose group(500 mg/kg/d),10 in each group.All the rats expcept those in NS group were intraperitoneal injection 35 mg/kg of PTZ perday,NP group were intraperitoneal injection the same times and dose of normal saline with the same method until the kindling criterion was reached(almost two weeks).All the rats expcept those in NP group and PTZ group were intragastric administration dose of the above three weeks,NP group were and PTZ group were intragastric administration the same times and dose of NS with the same method,The expression of Bax protein and Bcl-2 protein on the neurons of the hippocampus were analyzed by immunohistochemistry staining.Results:1:the normal control group had no seizure,the epileptic group induced by PTZ had seizure,and the six mediaction administration groups had seizure too,but the level of seizure were decreased.2:Compared with the PTZ gorup the lateneies of seizure were porlonged in the group of VAP durg positive group and OXC treatment group the difference had the significant meaning(p＜0.01),the level of seizure were decreased.OXC Low dose group medium dose group high dose group Compared with VPA Low dose group medium dose group high dose group the difference had the significant meaning(p＜0.01), the level of seizure were decreased.3:the NP group the expression Bax protein and Bcl-2 protein were low.Compared with the PTZ group in the VAP durg positive group OXC treatment group the number of the positive neurons of Bcl-2 protein were increased,the number of the positive neurons of Bax protein were decreased.VPA Low dose group was indifferent statistically Compared with OXC Low dose group(p＞0.05);medium dose group the difference had the meaning(p＜0.05);high dose group the difference had the significant meaning(p＜0.01).VPA Low dose group medium dose group and high dose group the difference had the significant meaning(p＜0.01);OXC Low dose group medium dose group and high dose group the difference had the significant meaning(p＜0.01).4:The Bcl-2\Bax ratio the NP group was indifferent statistically Compared with the PTZ gorup (p＞0.05);VPA Low dose group was indifferent statistically Compared with OXC Low dose group(p＞0.05);medium dose group high dose group the difference had the meaning (p＜0.01).VPA Low dose group medium dose group and high dose group the difference had the significant meaning(p＜0.01);OXC Low dose group medium dose group and high dose group the difference had the significant meaning(p＜0.01).Conclusion:1:The lateneies of seizure were prolonged and the levels were reduced in the epileptic rat models by VAP and OXC.2:OXC Low dose group medium dose group and high dose group was better than VAP Low dose group medium dose group and high dose group in the The lateneies of seizure were prolonged and the levels were reduced in the epileptic rat models.3:The VAP and OXC can protect hippocampal neurons in the epileptic rats induced by PTZ and can decrease the apoptosis in the hippocampal neurons after the seizure attacks.We supposed the mechanism may be related with enhanced theexpression of Bcl-2 protein and reduced he expression of Bax protein of the hippocampal neurons.4:The alteration of the Bcl-2\Bax ratio would play a vital role in regulating the neuronal apoptosis during epilepsy.5:OXC medium dosage group and high dosage group was better than VAP medium dosage group and high dosage group in the protect hippocampal neurons.6:VPA With the increase in the dosage to enhance in the protect hippocampal neurons.7:OXC With the increase in the dosage to enhance in the protect hippocampal neurons.