Clinical Significance Of CD24, VEGF, MVD Expression In Urothelial Carcinoma Of The Bladder

Objective: CD24 is a molecule that recently has raised considerable attention in tumour biology. It is involved in cell adhesion and metastatic tumour spread .In the mouse, CD24 was expressed in haematopoietic cell, thymocytes, brain and pancreas. In contrast to the mouse , CD24 was not expressed on human erythrocyte or thymocytes, but CD24 was high expressed in hematological malignancies as well as in several types of solid tumors. Recently studies revealed that high rates of CD24 expression detected in a variety of the most common tumours, such as B cell lymphoma, renal cancer, Merkel cell cancer, neura cell lineage tumors, pancreatic cancer, gastric cancer, colorectal carcinoma, breast cancer and so on. Moreover, high rates of CD24 expression was significantly associated with invasion metastasis ,relapse and prognosis, CD24 has been a marker of invasion metastasis ,relapse and prognosis in some malignant tumour.To investigation the clinical signifiance of CD24 and vascular endothelial growth factor (VEGF) expression in urothelial carcinoma of the bladder.Methods:To detect expression of CD24 and VEGF in 120 specimens(80 transurethral resection,40 radical cystectomy) of bladder urothelial carcinoma and 10 specimens normal bladder tissues by SP immunohistochemistry, Microvessel density(MVD) was figured out according to the positive staining for VEGF.Results:In normal urothelium,CD24 was localized to the cytoplasm of cell with very low intensity,and CD24 expression was higher in bladder urothelial carcinoma than that in the normal urothelium, the CD24 higher expression was significantly correlated with high tumor grade, high pTNM stage, lymph node metastasis(P < 0.05).The VEGF,MVD higher expression were significantly correlated with high tumor grade, invasion, lymph node metastasis.The expression of VEGF,MVD were increased with high pTNM stage. VEGF,MVD expression was higher in bladder urothelial carcinoma of invasion, lymph node metastasis than that of noninvasion,no lymph node metastasis,the expression of CD24 has positive correlation with the MVD(r=0.243, P<0.05).Conclusions: CD24 expression is obviously up-regulated in urothelial carcinoma was statistically significant, the expression of CD24 has positive correlation with the grade and stage of tumor,and it is correlated with the tumor invasion,metastasis and angiogenesis.The VEGF,MVD higher expression were significantly correlated with high tumor grade, invasion, lymph node metastasis.The expression of VEGF,MVD were increased with high pTNM stage, the expression of CD24 has positive correlation with the VEGF,MVD. CD24 and VEGF have a synergistic effect in bladder urothelial carcinoma angiogenesis.