Experimental Study Of Demyelination And Immunological Rejection Of Rats' Sciatic Nerve Allograft With The Tripterygium Glycoside's Pretreatment

ObjectiveThe experiment was designed to investigate the appropriate concentration of tripterygium glycoside by the experimental study of demyelination and immunological rejection of rats'sciatic nerve allograft with the tripterygium glycoside's pretreatment of different concentration so as to explore tripterygium glycoside's suppression.Methods1.Sixty SD rats(male,weighted 280±10g) which were regard as sciatic nerve allograft acceptor and were randomly divided into five groups(Group A,B,C,D andE,n=12).2.To repair the sciatic nerve gap of SD rats,the Wistar rats'sciatic nerve allografts about 1.5cm long were used with 24 hours'soak of different concentration of tripterygium glycoside.Experimental group A:200mg/Lgroup B:400mg/Lgroup C:800mg/L 3.The control groups(group D:the fresh sciatic nerve allograft from donors,group E:the fresh sciatic nerve allograft from themselves) were established.4.The morphological examinations(the observation of histology,light microscope,electron microscope),the detection of MBP(myelin basic protein)'s content and the study about CD4+,CD8+ T cells on the allografts in the acute phase were performed at the different time after operations.Results1.There were no significant differences among the experimental groups in morphology,the adhesions between allografts and connective tissue are better than group D.2.The allografts' morphous and the inflammatory cell infiltration are obviously better than group D;3.The degeneration of myelin sheath in Groups were at the different levels and there was not a significant difference between Group B and E(P＞0.05).4.The experimental groups had significant differences in immunological rejection compared with that in group D(P＜0.05).Conclusion1.To be an immunosuppressant,tripterygium glycoside can effectively suppress the acute immunological rejection after operations;2.Preserve the myelin sheath to some extent(especially with the concentration of 400ug/L,at the same level with Group E),in order to raise the probability of success about the peripheral nerve transplantations.