Effect Of Naoling Decoction On The Expression Of NR2B And BDNF In Hippocamp Of The Rat With Alzheimer Disease

Objective:To observe the effect of Naoling Decoction on ethology, hippocamal histomorphology,and expression of NR2B and BDNF in hippocamp of the rats with synthetic Alzheimer's disease,and explore the therapeutical effect on Alzheimer's disease rats and the potential mechanism of action of Naoling Decoction.Methods:Forty pure line SD(spragu-dawleg) rats were classified into five groups:normal group,sham- operated group,model group,Naoling Decoctio group and Piracetam group.The Alzheimer's disease model was established by Aβ1—42 injected into hippocamp in rats.The faculty of learning and memory was evaluated by Y-electric-maze test and Morris water maze experiment after establishing AD model.The brain tissues were removed to be made frozen sections and stained with congored to detect the aggradation of Aβ,the changes of cell morphology were detected by HE stain.Expression of NR2B,BDNF in hippocamp was measured with immunohistochemical staining.Results:(1)The results of Y-electric-maze test showed that compared with sham-operated group and normal group,model group's times to evade stimulate is more(p＜0.05);compared with model group,Naoling Decoctio group and Piracetam group's times to evade stimulate is less(p＜0.05).(2) The results of Morris water maze experiment showed that Compared with sham-operated group and normal group,the escaped and latent period of model group was delayed significantly(p＜0.05).In exploratory experiment, the average pass through times in sham operation group and normal group were more than that of other three ones,and that of the model group was lest, there were significant difference(p＜0.05).(3)stains:Congo red coloration result:in the hippocampal fields of rats in sham-operated group and normal group,orange amylaceous aggradation can't be seen.In the hippocampal fields of rats in the model group significant orange amylaceous aggradation can be seen in the area that injecting Aβ1—42,this denota there is aggradation of Aβin the hippocampal fields of rats in the model group.HE stain:In AD model rats group,the number of neuron in hippocamp was less and the array of neuron was more irregular than those in Naoling Decoctio group and Piracetam group.(4)The result of NR2B with immunohistochemical staining denote:The compasion of the gray scale value about NR2B in hippocamp of each group,the model group were lower than sham-operated group and normal group(p＜0.05);the gray scale value of Naoling Decoction group and Piracetam group were higher than the model group's gray scale value(p＜0.05).(5)The result of BDNF with immunohistochemical staining denote:The compasion of masculine cells about BDNF in hippocamp of each group,the model group were less than sham-operated group and normal group(p＜0.05);the Naoling Decoction group and Piracetam group were more than the model group(p＜0.05).Conclutions:(1)The model which was established by Aβ1-42 injected into hippocamal was best,because the model rats had the changes of praxiology and pathology.(2)The learning and memory of the model rats were decreased obviously,Naoling Decoction can improve the learning and memory of the model rats.(3)Naoling Decoction can dcrease neuronal loss and better pathomorphological changes in hippocampal of AD rats.(4)Naoling Decoction can elevate the expression of NR2B in hippocamp of AD rats, Elevate the expression of BDNF,those partly may be therapeutic mechanism on Alzheimer's disease of Naoling Decoction.