Effect Of Naoling Decoction On The Expression Of IL-1β And TNF-α MRNA Of The Rat With Synthetic Alzheimer Disease

Background and Objects:Alzheier's disease,a centra neurode-generative disease which is characterized by evident learning,memory and cognitive function impairment.The pathology characteistics are the depo-sition outside the neuron cell of senile plaque(SP) which is mainly composed of P-amoyloid protein,inside the neuron cell of neurofibrillary tangles and the absence of neurons and axons in hippocampus,cerebral area.Although it's etiopathogenisis and mechanism is still unknown,neuro- inflammation from deposition ofβ-amoyloid has been accepted as a pathological core for Alzheier's disease.There are many Chinese crude drugs which are good for the therapy of AD.This text approach Nao-ling Decoction take part in the change of AD model rats'learning,memory and pathological, and the effect on IL-1βand TNF-αmRNA,which lays the foundatian for further elucidating its mechanism and treatments for AD.Methods:Thirty SD (spragu-dawleg) rats were classified into five groups after Y-electric-maze testing:a normal group, a sham operated group, a model group, Naoling Decoctio group and Piracetam group. The Alzheimer disease model was established by Aβ1-42 injected into hippocamal in rats. The faculty of learning and memory was evaluated by Y-electric-maze test and Morris water maze experiment after establishing AD model. The brain tissues were removed to be made frozen sections.Expression of IL-1βand TNF-a mRNA in CA3 region was measured with in situ hybridization.Results:(1)The results of Y-electric-maze test showed that compared with sham-operated group and normal group,model group's times to evade stimulate is more(p<0.05);compared with model group,Naoling Decoctio group and Piracetam group's times to evade stimulate is less(p<0.05). Mean-while,there existed no obvious difference between these two therapy groups (p>0.05).(2)The results of Morris water maze experiment showed that Com-pared with sham operated group and normal group,the escaped and latent period of model group was delayed significantly(p<0.05).In exploratory experiment,the average pass through times in sham operation group and normal group were more than that of other three ones,and that of the model group was lest,there were significant difference (p<0.05).(3)The result of IL-1βand TNF-a mRNA with in situ hybridization denote:The compasion of the gray scale value about IL-1βand TNF-αin hippocampal CA3 of each group,the model group were lower than sham-operated group and normal group (p<0.05);the gray scale value of Naoling Decoction group and Piracetam group were higher than the model group's gray scale value(p<0.05).Conclutions:(1)The presentation of results of the study that the model was established by Aβ1-42 injected into hippocamal was blest,because the model rats had the changes of praxiology and pathology. (2)The learning and memory of the model rats were decreased obviously,Naoling Decoction can improve the learning and memory of the model rats.(3) Naoling Decoction can weaken the expression of IL-1βand TNF-αmRNA in hippocampal CA3 of AD rats,those partly may be therapeutic mechanism on Alzheimer disease of Naoling Decoction.