Expression Of BRMS1 Protein And CA Ⅰ Protein And Their Clinicopathological Significances In Primary Hepatic Carcinoma

Objective To investigate the expression of Breast Cancer Metastasis Suppressor 1(BRMS1) protein and Carbonic Anhydrase I(CA I) protein in Primary Hepatic Carcinoma(PHC),and to explore the relationships of themselves and the correlation between them and the clinicopathological characteristics of PHC.Methods The specimens from 47 patients with Primary Hepatic Carcinoma(PHC) and 20 corresponding paraneoplastic hepatic tissues were fixed in 10%formalin and routinely embedded in paraffin.The specimens were continuously sliced into 4μm-thick sections.Envision^TM immunohistochemistry was performed to detect the expressions of BRMS1 protein and CA I protein with monoclonal antibodies and scored them under high-power microscopy.Results In the 47 Primary Hepatic Carcinoma(PHC) cases,there are significantly statistical distinctions in the expressive positive rate and the expressive scores of BRMS1 and CA I protein between cancer tissues and paraneoplastic tissues(P＜0.01,P＜0.05)The cancer tissues with intrahepatic metastasis and tumor thrombosis have significantly lower expressive positive rate and expressive scores of BRMS1 protein than the others(P＜0.01,P＜0.05),and also there is a sinificantly lower positive rate in nodular and diffuse PHC than massive ones(P＜0.05).But we haven't found the relatioships between BRMS1 and other main clinicopathological characteristics of PHC(P＞0.05).There is no correlation between the expression of CA I protein and clinicopathological characteristics of PHC,except that the expressive positive rate and the expressive scores of PHC significantly gradually increase with the descending differentiation degree(P＜0.05).There is no evident correlation between the expressive scores of BRMS1 protein and that of CA I protein in cancer tissues of PHC(r= -0.37,P＞0.05)Conclusion The lost expression of BRMS 1 protein in PHC plays an impotant role in the invasive and metastatic process,without effect on the transformation and growth of PHC.On the contrary,the overexpression of CA I protein in PHC is closely related to the differentiation,and it may have nothing to do with the growth,invasiveness and metastasis.And there is no correlation between the expressive scores of BRMS1 protein and that of CA I protein in cancer tissues of PHC...