| Background: That atherosclerosis (AS) result in coronary heart disease(CHD) acutely threatens human being's health, and bring heavy burden to the family and society. Research display that AS is an especially kind chronic inflammation process, and intercellular adhesion molecule-1(ICAM-1)associates with this process closely. ICAM-1 is a member of the immunoglobulin superfamily, and express in vascular endothelial cell(VEC),monocaryon(MN),leukomonocyte(LY) etc. ICAM-1 commonly expresses lowly or doesn't express even. In the development process of AS chronic inflammation, MNs adhere to ECs mostly by ICAM-1. Adhesion molecules out of cell can be resolved by especial enzymes and detected in blood serum that are called dissolubility adhesion molecule. In Chinese traditional medicine"XIONGBI"corresponds to CHD of Western medicine. The traditional Chinese medicine is good for the patients of CHD, but the mechanisms isn't clear .This study is to explore the effect of Faeces Trogopteri (FT) aqueous extract on the expression of intercellular adhesion molecule (ICAM-1) and endarteropathy and its anti artherosclerosis (AS) in the experimental AS rats, and enhance the rationale of TCM for CHD.Methods: Sixty healthy male clean six weeks Wistar rats were randomly divided into 4 groups of 15 rats per group: blank, model, FT low dose(FTL, 2.5 g/kg weight) and FT high dose (FTH, 10 g/kg weight) groups. The blank group was fed standard feedstuff, the other three groups were duplicated by feeding high cholesterol diet and peritoneal injecting vitamin D3 6 weeks later, the serum soluble intercellular adhesion molecule-1 (sICAM-1) level in the rats was measured by enzyme liked immunosorbent assay kit; the expression of ICAM-1 proteinum in the endarterium of AS rats was detected by immunohistochemical technique; the pathological changes in the endarterium of AS rats was observed by transmission electron microscope.Results: The rat AS model was successfully duplicated. Compared with the other groups, the serum sICAM-1 level of blank group was markedly lower〔( 3.10±0.42 ) vs (81.62±7.30),(82.26±6.72) ,(82.41±7.06) ng/ml,P<0.01〕; after the FT interfering, the serum sICAM-1 level of FTL and FTH group rats was markedly lower〔(42.73±6.97) vs (82.26±6.72), (21.29±4.27) vs (82.41±7.06) ng/ml,P<0.01〕, and lower than that of model group〔(42.73±6.97) vs (85.63±11.43), (21.29±4.27) vs (85.63±11.43) ng/ml, P<0.01〕. The expression of ICAM-1 proteinum at the endarterium of FTL and FTH group rats was obviously fewer than those of model group, and those of FTL group was fewer than those of FTH group. Transmission electron microscope result showed hat the endothelial damage of the aortas in FTL and FTH groups was less severe than that in model group.Conclusion: 1.The AS model of rat can be made by feeding HFD and injecting vitamin D3,but the prescription of HFD may need to be improved.2. The serum soluble intercellular adhesion molecule-1 (sICAM-1) level in the AS rats is conspicuously higher than that of the blank group, which indicates that sICAM-1 correlates with AS inflammation process closely.3. FT can downregulate the expression of ICAM-1 in the artery of experimental AS rats, and lighten the endothelial damage of the aortas, higher doe(s10 g/kg weight) is better than lower dose(2.5 g/kg weight), which may account for the anti AS effects of FT. |
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