| PurposeAntibacterial eye drop is often topically utilized in clinic before ocular surgery, but the standard in the application of antibacterial eye drops topically in preoperative period has not been reported. Current study was to quantitatively analyze the concentration of levofloxacin gutta in aqueous humor after topical administration before cataract surgery.MethodsTotal 90 patients undergoing cataract surgery were divided into A, B, C groups randomly. The levofloxacin gutta was topically used once per 6 hours for 1 day before cataract surgery in group A, and levofloxacin gutta was used once per 4 hours for 1 day before cataract surgery in group B and once per 6 hours for 3 day before surgery in group C. The eye drops was ended before one hour of cataract surgery in all patients. 0.1 mL of aqueous humor sample was collected from every patient during the cataract surgery and was preserved in refrigerator with the tempertura of -20. Levofloxacin gutta level in aqueous humor was determined using a validated high-performance liquid chromatography.ResultsConcentration of levofloxacin gutta in aqueous humor was ( 0.68±0.58 )μg/mL, ( 1.05±0.66)μg/mL and ( 1.89±0.98)μg/mL in group A, group B and group C respectively, showing a significant difference among these three groups(F=24.186, P<0.001). Compared with group A, the levofloxacin gutta level in aqueous humor was significant higherp(p=0.043,p=0.000) , and that in C was evidently increased in comparison with group B(p=0.000).ConclusionThere is a positive correlation between concentration of levofloxacin gutta in aqueous humor and administration times of levofloxacin gutta before surgery.
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