The Research Of Serum Tumor Biomarkers For Pancreatic Carcinoma By Comparative Proteomics

Pancreatic carcinoma (PC) is one of the malignant tumor, its clinical manifestations was not obvious, developed rapidly, and its prognosis is very bad. At present, there is no effective screen and early diagnostic methods. Current serum tumor biomarkers for pancreatic carcinoma including carbohydrate antigen 19-9 (CA19-9), carcinoembryonic antigen (CEA) and carbohydrate antigen 50 (CA50) all have many defects such as bad-sensitivity, bad-specific, high rate of false-positive, they could not play their due role in the early diagnosis of pancreatic carcinoma. Surgical management is only effective method to treat pancreatic carcinoma, but metastasis and local recurrence were more easily appeared in patients of pancreatic carcinoma after surgery. It is necessary to identify the serum markers for pancreatic carcinoma in order to improve early diagnosis rate and predict recurrence and metastasis. In this study, fluorescence 2-D Difference gel electrophoresis (2D-DIGE) and surface enhanced laser desorption / ionization time-of-flight mass spectrometry(SELDI-TOF-MS) were be used to analyze the expression of serum protein in the pancreatic carcinoma patients ( preoperative group and postoperative group), chronic pancreatitis (CP) patients and healthy control group. Differentially expressed proteins of pancreatic carcinoma were be identified.ObjectiveTo identify differentially expressed proteins of pancreatic carcinoma by comparative proteomics, which provide reference to the screenings of serum tumor markers for pancreatic carcinoma. At the same time differential proteins which were associated with recurrence and metastasis of pancreatic carcinoma were also be identified, it would provide a theoretical foundation for improving early diagnosis of recurrence and metastasis of pancreatic carcinoma as well as forecasting prognosis of pancreatic carcinoma.Methods(1)proteins were separated from peripheral blood in pancreatic carcinoma patients (preoperative group and postoperative group), chronic pancreatitis patients and normal control group by 2D-DIGE, differentially expressed proteins spot were analyzed and described.(2)Several proteins were be identified from the differentially expressed protein spots by SELDI-TOF-MS. It was confirmed that there were closely relationships between those proteins and pancreatic carcinoma, including the occurrence, recurrence and metastasis of pancreatic carcinoma.Results(1)Seven differentially expressed proteins: complement component 3(C3), cDNA FLJ50830( highly similar to Serum albumin), haptoglobin protein (HP protein), apolipoprotein E (ApoE), C4B1 (Homo sapiens), Isoform 1 of TNFAIP3-interacting protein 2(TNIP2)and Chain A (The Structure Of Pentameric Human Serum Amyloid P Component) were identified by 2D-DIGE and SELDI-TOF-MS.(2)C3 and cDNA FLJ50830 were high expression in pancreatic carcinoma group(including preoperative group and postoperative group) compared with normal control group. The expression of C3 was higher in the preoperative group of pancreatic carcinoma than in the chronic pancreatitis group, the expression of cDNA FLJ50830 was higher in chronic pancreatitis group than in the preoperative group of pancreatic carcinoma.(3) cDNA FLJ50830, HP protein, ApoE,C4B1,Isoform 1 of TNIP2 and Chain A (the structure of pentameric human serum amyloid P component) were higher expression in the preoperative group compared with the postoperative group of pancreatic carcinoma.Conclusions(1)Inflammatory response regulatory factor C3 was high expressed in pancreatic carcinoma compared with normal control group revealed that inflammatory response was closely related to the occurrence and development of pancreatic carcinoma, which would provide a new way to understand the pathogenesis of pancreatic carcinoma.(2)cDNA FLJ50830 was a newly discovered protein differentially expressed in pancreatic carcinoma, which may become a new serum marker for the differential diagnosis of pancreatic carcinoma and chronic pancreatitis.(3)HP, apolipoprotein E, C4B1, Isoform 1 of TNIP2 and Chain A (the structure of pentameric human serum amyloid P component ) may be the serum tumor markers which could diagnose the recurrence and metastasis of pancreatic carcinoma.