Expression And Clinical Significance Of TRF1, TRF2 And HPV 16/18 In Human Squamous Cell Carcinoma Of The Uterine Cervix And Cervical Intraepithelial Neoplasia

Objective To investigate expression and clinical significance of TRF1,TRF2 and HPV 16/18 in human squamous cell carcinoma of the uterine cervix and cervical intraepithelial neoplasiaMethods The infection rates of HPV16 /18 in 15 cases of normal cervices, 36 cases of cervical intraepithelial neoplasia (CIN) and 35 cases of squamous cell carcinoma of the uterine cervix were detected by hybridization in situ. Expression of TRF1 and TRF2 was detected by immunohistochemical staining in these groups.Results The infection rates of HPV16 /18 in normal cervical epithelium, CIN and squamous cell carcinoma of the uterine cervix were 20.0%(3/15), 63.9%(23/36) and 97.1%(34/35) respectively. There were significant differences among these cervical lesion groups and the normal cervical epithelium (P < 0. 01). The positive rates of TRF1 in normal cervical epithelium, CIN and squamous cell carcinoma of the uterine cervix were 86.7% (13/15), 63.9% (23/36) and 40.0% (14/35) respectively. There were significant differences among squamous cell carcinoma group and CIN, the normal group. The positive rates of TRF2 in normal cervical epithelium, CIN and squamous cell carcinoma of the uterine cervix were 33.3% (5/15), 52.8% (19/36) and 80.0% (28/35) respectively. TRF2 expression was higher in squamous cell carcinoma than that in CIN and the normal cervical epithelium (P < 0. 001). TRF1 expression was negatively correlated to HPV 16/18 infection (Rs = -0. 302, P <0.05). TRF2 expression was positively correlated to HPV 16/18 infection (Rs= 0. 452, P < 0. 01).Conclusion TRF1 and TRF2 play a role in the progress of the human squamous cell carcinoma of the uterine cervix. Up-regulation of TRF1 and down-regulation may be related to the infection of HPV16 /18.