Impact Of Nutritional Obesity On Serum Leptin, Insulin And Ovary Of Mice In Development Period

Object:to discuss relationship between obese and ovary of mice in development period,we made obesity model by high fat diet and observe serum leptin,insulin levels and ovary pathology changes.We also identify impact of serum leptin and insulin in the process of mice ovary development.It is obvious that rational diet is necessary in preventing reproductive system disease.Methods:We made mice obesity model with high-fat diet,then monitored leptin,insulin concentration and ovary changes.Healthy Kunming mice were divided randomly into 2 groups(n=10 in each group).The control group:the mice were fed by normal food.Experimental group:the mice were fed by diet rich in fat and sugar. After 40 days of feeding,the body weight of mice was determined,and the average increasing rate of body weight in each group was calculated and normalized with food intake.Meanwhile,serum leptin and insulin level was measured by ELISA method;growth of ovary,leptin receptor were measured by immunohistochemistry method,then we calculation IOD of leptin receptor.Results:(1)Comparing with normal mice,obesity mice ate similar quantity of food(P＞0.05),but obesity mice took in more energy(P＜0.01). Differentiation was found on body weight after 20 days(P＜0.05).40 days later,the obesity mice are heavier(P＜0.01).The obesity mice were 8 and the other 2 were not,which meant obesity model were made successfully. (2) The serum leptin and insulin concentration of obesity mice were much higher than normal mice(P＜0.01 P＜0.05).(3) Ovaries in obesity mice were bigger and harder than norm.We watch sections under microscope: norm ovaries have achieved complete puberty;they have been ovulation, corpus luteum and all levels follicles are exist in ovaries.The ovaries in obesity mice grew slower and have more follicles,which including more primary atresia follicles and less mature follicles.We also found a little corpus luteum in obesity mice.(4) There are significant increases in serum leptin,insulin level and leptin receptors of 7 mice found ovary abnormalities than mice in control group(P＜0.01,P＜0.05,P＜0.05).Conclusion:In our experiment,high fat diet can induce nutritional obesity in some degree;this degree is influenced by quantity of intake energy.Obesity which induced by high fat diet may disturb reproductive hormone secretion which may cause abnormal secretion of leptin and insulin and make their secretion increased.At the same time,it suggests us insulin and leptin can interact each other.Obesity can induce abnormalities in ovaries which show PCO changes by affect leptin and insulin secretion level.