Seperation And Isolation Of Active Constituents And Antioxidant Activity Of Extracts From Daphne Odora Var.marginata

Daphne odora var.marginata,family of Thymelaeaceae,is a famous ornamental flower and medical plant,also used as paper making and aromatic oil extracting.The pounded leaves could applied at the afflicated part,utilized as ulcerate,gall and mastitis treatment.After marinated in wine,the flowers obtain the ability to cure toothache,rheumatoid arthritis,gall caused by gall and verious ache.Recent pharmacological studies indicated that Daphne odora var.marginata had anti-lipid peroxidation,analgesic,immunoloregulation,anti-inflammation,antibacterial, anti-hypoxia and anti-tumor bioactivity.In this thesis,separation and purification of the chemical constituents and assaying about the antioxidant activity of Daphne odora var.marginata were studied.For the sake of founding the theoretical foundation for the depth development and utilization of Daphne odora var.marginata.The thesis were divided into six chapters,the main contents of each chapter was as follows:Chapter 1.Introduction of the mechanism and application of five antioxidant and free radical scavenging assays;summarize about the chemical constituents and medical activiteies of daphne odora var.marginata.Chapter 2.Extracting the crude plant of daphne odora var marginata by normal solvent with partition.Chapter 3.Assayed the activities of daphne odora var.marginata by using superoxide radical scavenging,DPPH radical scavenging,hydroxide radical scavenging,ferrous ion chelating and ferric ion reducing.Measured the total flavonoid contents of each fraction,compared the activities with the total flavonoid contents.Chapter 4.Seperation and purification of the acetic ether extract with high activity by silica gel column chromatography after choosing eluent by TLC,three subfractions were collected. The chemical constituents of EA and its subfractions were performed on RP-HPLC.Chapter 5.Six chemical and biological assays were performed on evaluting the activities of EA and its three subfractions.They were superoxide radical scavenging,DPPH radical scavenging,hydroxide radical scavenging,ferrous ion chelating,ferric ion reducing,protective effect on damaged DNA caused by hydroxide radical.Investigated the coefficient between chemical constituents and contents and verious activities.The total flavonoid and phenolic contents were also measured,and their coefficient with different activities were estimated too.Chapter 6.Isolation and purification the active fraction F2 by sephadex LH-20 column chromatography,two active compounds obtained.It need a furder structural identification and bioactive detection.Chapter 7.Summarized the entire experimental results.Prospected the experiment and research in the future.