Isolation And Identifjication Of Chemical Constituents In The Ethanol Extracts From Daphne Odora Var. Margirmta And Studies On Their Antioxidant Activities

Daphne odora var. marginata, family of Thymelaeaceae, is a famous flower and medical plant in the regions along the Yangzi River. It has been used in Chinese traditional medicine for many diseases. Their leaves have been mashed up to kurashiki the affected part for the ulcerate, injury, and mastitis; Their flowers soaked by white spirit have been used to cure rheumatoid arthritis, rheumatism, carbuncle, furuncle, miliaria, unknown toxin and traumatic injury. In this dissertation, the chemical constituents and antioxidant activity of the extracts from Daphne odora var. marginata were studied to find the main medicinal compounds with biological activity or novel structure. It would offer the theoretical foundation for the depth development and utilization of Daphne odora var. marginata.In the first chapter, we have described the progress of chemical constituents and pharmacological activity in Daphne odora var. marginata. Furthermore, the distribution and bioactivity of coumarin and daphnodorin which were characteristic components in Thymelaeaceae were reviewed.In the second chapter, the fractions was from Daphne odora var. marginata extracted by 70% ethanol, and then were subjected to column chromatography on silica gel, ODS and Sephadex LH-20. Ten compounds were separated and purified from this plant.In the third chapter, the physical and chemical properties of the ten compounds were investigated by UV-vis, IR, ESI-MS, EI-MS,1H NMR and 13C NMR, as follow: wikatrols B, kaempferol, daphnodorin B, daphnodorin D2, daphnodorin A, daphnodorin C, daphnodorin D1, daphnodorin I, daphnetin, daphnoretin. This research showed great potential applications in medicinal plant.In the fourth chapter, the pertinence relation between structural and the antioxidant activities of nine compounds which separated and purified from this plant was respectively studied by the reducing power and scavenging activity of DPPH radicals, total antioxidant activity, and OH- scavenging activity. Therefore, it would provide scientific foundation for curing disease related to radicals by using these compounds as safe, effective antioxidants or medicine.