Studies On Structural And Functional Character Of Ganoderma Lucidum Polysaccharides

Ganoderma lucidum polysaccharides is one of the main effective ingredients in Ganoderma lucidum,many pharmacological and clinical research show that it is a non-toxic side effects on normal cells,it can activate the immune cells to enhance immune function of regulator,with lowering blood sugar,anti-tumor,anti-oxidation and other biological activity.Ganoderma lucidum primary polysaccharides were distilled and prepared from Ganoderma lucidum cultured in three different conditions,and the purity of primary polysaccharides was also detected.The results showed that the content of polysaccharides in novel technical polysaccharides,culturing fruit body and wild fruit body was 2.1%, 0.16%and 0.17%.The polysaccharides' purity of novel technical polysaccharides, culturing fruit body and wild fruit body was 94.6%,94.8%and 95.2%,respectively.There were no proteins or nucleic acids in polysaccharides through UV spectra analysis.Three kinds of polysaccharides all had infrared characteristic absorption peaks by use of IR spectra,and the results of fingerprint region revealed that polysaccharides of mycelium might contain a-pyran glucose and polysaccharides of fruit body might containβ-pyran glucose.In vivo and in vitro anti-oxidation activities of mycelium polysaccharides were measured.In experiment in vitro,the anti-oxidation activity was detected through the ability of Ganoderma lucidum polysaccharides clearing·OH using salicylate-Fe²⁺-H₂O₂ systems.In experiment in vivo,it was detected with the effect of Ganoderma lucidum polysaccharides on GSH-Px and superoxide dismutase(SOD) in blood serum and liver. The results showed that Ganoderma lucidum polysaccharides exhibited a certain level of were able to reducing power and were able to clear·OH at a certain level in vitro,and in vivo,Ganoderma lucidum polysaccharides could dosage-dependently increased the activities of GSH-Px and SOD in blood serum and liver.The effect of Ganoderma lucidum polysaccharides on relieving the physical fatigue of mice was also studies through measuring the loading swimming time of mice,the content of hepatic glycogen,blood urea nitrogen and lactic acid of mice.The results showed that compared with control group,the loading swimming time of the high, medium and low dose groups increased by 90.2%,55.7%and 55.7%,respectively,the content of liver glycogen increased by 31.1%,45.4%and 43.9%,respectively,and the Compared with control group,was reduced by 36%,10.6%and 8.5%,respectively.All the results revealed that Ganoderma lucidum polysaccharides could alleviate fatigue of mice.