Association Of The Monocyte Chemoattractant Protein-1 Gene Polymorphisms With Acute Coronary Syndrome

Backgroud Acute coronary syndrome(ACS) is a spectrum of serious and developing Coronary heart disease(CHD),including unstable angina pectoris(UAP),ST-segment elevation myocardial infarction(STEMI) and non-ST-segment elevation myocardial infarction (NSTEMI).ACS occurs when unstable atherosclerotic plaque ruptures,ulcerates and blood platelet assembling,thrombus formation,which results in sudden total or near-total arterial occlusion and myocardial infarction.CHD/ACS is a complex multifactorial disorder which is believed to result from the interplay between a person's susceptible genetic makeup and various environmental factors.A positive family history of CHD is a strong independent predictor of CHD risk.Family studies have shown that the risk of developing CHD/ACS is 2 to 12 times higher for individuals with a positive family history compared with those without a family history of CHD.The heritable basis is increasingly recognized as a crucial component in the formation and rupture of atheromatous plaques,and thrombosis.The monocyte chemoattractant protein-1(MCP-1),also known as CCL2,is a CC-type chemokine that is thought to be responsible for monocytes and T-lymphocytes recruitment in inflammatory conditions.MCP-1 levels are also elevated in the systemic circulation of patients with acute coronary syndrome.There is genetic evidence in man to support a functional role for MCP-1 in atherogenesis.The SNP -2518G in the regulatory region of MCP-1,which causes increased promoter activity,is associated with elevated,circulating levels of MCP-1 and increased risk of myocardial infarction.However,the results of the role of the MCP-1 gene polymorphism and its plasma levels in MI are controversial.For the reason that a case-control study design was adopted in present study to explore the possible involvement of MCP-1 in MI in a Han Chinese population.Objective To investigate the impossible association between the monocyte chemoattractant protein-1(MCP-1) gene A-2518G single nucleotide polymorphism(SNPs) in the promoter region with acute coronary syndrome(ACS) in Chinese Han population of Sunan region.Methods This study was conducted with a case-control design in 484 ACS patients including 290 acute myocardial infarction(AMI) patients and 194 patients with unstable angina pectoris(UAP) and 346 control subjects who were be free of coronary by Coronary Angiography checks(control group),including 166 patients with coronary atherosclerosis and 180 subjects who were not stenosis in coronary.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used for the detection of the A-2518G polymorphism in MCP-1 gene,and then the frequency of genetype was statistically analyzed.Results There were AA,AG and GG genotypes of MCP-1 gene A-2518G polymorphism in the ACS group and control group,The two groups could be considered as a genetic equilibrium representative by Hardy-Weinberg equilibrium(P＞0.05),Compared with the control group,the frequencies of AA(15.32%vs 16.12%),AG(53.47%vs 51.86%), GG(31.21%vs 32.02%) and G(57.95%vs 57.95%) allele genotype were not significantly different in ACS group(P was 0.083,0.673,0.821 and 1.00,respectively).Multivariate logistic regression analysis indicates gender,age,smoking,diabetes,TG and LDL-C that there was no significant correlation between MCP-1 gene A-2518G polymorphism with ACS (P＞0.05).There was no significant difference between two groups of gender and early age (P＞0.05).There was no significant difference between the AMI group and the UAP group with the normal coronary group in the frequencies of AA,AG and GG genotype and G allele genotype(P＞0.05).Conclusion Our date shows that MCP-1 gene A-2518G polymorphism was not associated with the risk of ACS in the Chinese Han population of Sunan region.