Experimental Study Of Edaravone Protects Against Small Intestinal Ischemia-Reperfusion Injury In Rats

[Objective]To study the protective effects of edaravone against ischemia-reperfusion injury of small intestine in rat intestinal mucosa damage,inflammatory factorand anti-inflammatory factor release in order to find a way to protect clinical small intestine from I/R injury.[Methods]Sixty SD rats weighted about 280g-320g were divided into three groups randomly:control group,I/R group,edaravone group(n=20).Each group was divided into two subgroups according to time of reperfusion (n=10),The control group:the rats underwent a 3cm medium length-wise laparotomy,exposure of the small intestine,identification and dissection of the SMA and then,closure abdominal cavity.The I/R group:the rats were operated by occlusion of SMA for 45 min,and then,same with control group. The edaravone group:the rats were operated by occlusion of SMA for 45 min, 5 min before reperfusion,inject edaravone through tail vein(3mg/kg),and then,same with I/R group.At the end of the operation,the rats were sacrificed,and dissected 10cm segment of ileum to determination of intestines mucous membrane's injury.The serum of TNF-α,IL-1β, IL-6,IL-10 are measured by ELISA.Cut tissues from liver and spleen at the same weight and portal vein blood to detect bacteria.[Results](1)The changes of intestinal mucosa:In I/R group,the intestinal mucosa presented dilated and exposed capillaries and denuded villi,some of villi hemorrhage,ulceration.The lamina propria was edema and infiltrated with inflammatory cells.The intestinal mucosal structure maintain intact with lifting of the epithelial layer from the lamina propria and moderate extension of the subepithelial space in edaravone group.In edaravone group,histopathologic scores were significantly lower than that of I/R group.(2) Compared with Contraol group,the serum contents of TNF-α,IL-1β,IL-6 in I/R group,edaravone group are obviously higher, and the edaravone group is lower than that of I/R group.The contents of IL-10 in other two groups are lower than that of control group,and edaravone group are higher than that of I/R group.(3)Compared with control group, the bacteria transposition in I/R group,edaravone group is obviously higher,and the edaravone group is lower than that of I/R group.[Conclusion]Edaravone can protects against small intestinal ischemia-reperfusion injury in rats.(1)Edaravone makes small intestinal mucosal damage degree alleviated in rat I/R injury.(2) Edaravone can reduce the contents of inflammatory TNF-α,IL-1β,IL-6 and promote the contents of anti-inflammatory medium Ih-10 in rat I/R injury.(3) Edaravone can reduce the bacterias transposition of small intestine in rat I/R ingury.