Study Of The Relationship Between Zanthoxylum Nitidum DC. On Cancer Cells NCI-H460 And SK-OV-3 Fingerprint And Pharmacodynamics

Objective:Zanthoxylum nitidum DC. and cancer cells NCI-H460, SK-OV-3 were studied and the fingerprint was established. On the basis of the Zanthoxylum nitidum DC. HPLC fingerprint and its anti-tumor activity, backward-elimimation procedure were applied to find the correlation between Zanthoxylum nitidum DC. fingerprint and pharmacodynamics.Methods:1.Screening of the part of Zanthoxylum nitidum DC. with antitumor activity:Five different polar dissolvants:methanol, ethanol, acetoacetate, chloroform and ligarine were used to abstract the Zanthoxylum nitidum DC.. MTT was used to detect the extracts of Zanthoxylum nitidum DC. on NCI-H460 and SK-OV-3 in vitro.2. Study on the anti-tumor activity of Zanthoxylum nitidum DC.:The cytotoxicity of Zanthoxylum nitidum DC. from 43 metropolis on NCI-H460 and SK-OV-3 were detected by MTT assay in vitro.3. The estabishment of Zanthoxylum nitidum DC..HPLC fingerprint: According to the requirements of the chemical fingerprint, estabish the HPLC fingerprint of Zanthoxylum nitidum DC.. The similarity evaluation of chromatograms was processed by chromatographic fingerprint evaluation system software which promulgated by Pharmacopoeia Commission.4. Study on the correlation between characteristic of Zanthoxylum nitidum (Roxb.)DC. fingerprint and pharmacodynamics:at the premise of the acquired quantitateve fingerprint characteristic and pharmacodynamic data, backward-elimimation procedure was applied to find the correlation between Zanthoxylum nitidum DC. fingerprint and pharmacodynamics.Results:1. Screening of the part of Zanthoxylum nitidum DC. with anti-tumor activity:The active ingredient of Zanthoxylum nitidum DC. in anti-tumor activity mainly in the chloroform part. The chloroform part contained nitidine chlorine, liriodendrin and other alkaloids.2. The extracts of Zanthoxylum nitidum DC. had significant inhibitory activious in vitro on NCI-H460 and SK-OV-3.3. The estabishment of Zanthoxylum nitidum DC. HPLC fingerprint:The Zanthoxylum nitidum DC. HPLC fingerprint could comprehensive response to the distribution characteristics of the main component.4. It could be concluded that there were 15 peaks have effective on NCI-H460 in the Zanthoxylum nitidum DC. HPLC fingerprint. The equation of the fingerprint and pharmacodynamics:Y=32.703-12.216X2-8.102X4+13.553X5 +15.953X6-3.802X8+10.979X9-9.449X11+10.503X14-6.900X15-3.519X16+11.878 X17+14.683X18-8.121X21-8.482X22+4.737X23 (R2=0.923); There was 15 peaks had effective on SK-OV-3 in the Zanthoxylum nitidum DC. HPLC fingerprint. The equation of the fingerprint and pharmacodynamics:Y=74.908+18.784X4--10.686X5+10.615X6+2.889X8-5.170X10-7.046X11-7.498X12+4.217X13-8.057X15-9.265X18-15.088X20-4.015X22+3.356X7+5.175X16-2.424X24 (R2=0:938). It showed that the calculated value were very close to the actual value compared to the datas of 10 origins Zanthoxylum nitidum DC.Conclusions:The equation of the Zanthoxylum nitidum DC. fingerprint and pharmacodynamics is significant statistically. The cytotoxicity can be estimated through the equation of the Zanthoxylum nitidum DC. fingerprint and pharmacodynamics.