The Expression Of Ezrin And RhoA In Nasopharyngeal Carcinoma And Clinical Signification

Objective:To investigate the expression of Ezrin and RhoA proteins in nasopharyngeal carcinoma (NPC) tissue in different clinical stages, and anlyzing their functions and relationships in the NPC carcinogenesis and development mechanism. The purpose were:A. to study their roles in invasion and metastasis of NPC. B. to provide the new theoretical foundation and to produce new valuable indicators for the early diagnosis and the judging prognosis of NPC.Methods:1. Specimens paraffin-embedded tissue of 150 NPC with complete clinical data were collected as experiment group, and 20 cases of nasopharyngeal mucosa chronic inflammation paraffin-embedded tissue were chosen to be compare group.2. Utilize Supervision two-step immunochemical methods to detect expression of Ezrin and RhoA proteins.3. SPSS(13.0) statistical software was applied for data processing to analysis:A. the correlation between the expression of Ezrin and RhoA proteins and the major clinical pathological characteristics of NPC (Chi-square test and Spearman rank correlation). B. the prognostic relevance between the expression of Ezrin and RhoA proteins and the Post-treatment survival period of NPC, the survival rate was caculated by Kaplan-Meier method and compared by log-rank test.Results:1. The positive expression rates of Ezrin and RhoA proteins in NPC tissue were 74.7% and 62.7% and nearly no positive expression in nasopharyngeal mucosa chronic inflammation tissue. There was a statistically significant difference (p<0.001).2. In TNM stages, the positive expression rates of Ezrin and RhoA proteins in NPC tissue which were 29.4%,65.5%,92.3%,88.5% and 17.6%,52.7%,78.8%,80.8% in T1,T2,T3,T4 stages, There were statistically significant difference between every stages (P<0.05 or P<0.001) besides T3 and T4 stage; 53.8%,71.4%,79.6%,87.9% and 46.2%,54.8%,65.3%,81.8% in No, N1, N2, N3 stages, There were statistically significant differenc between No and N2, No and N3, N1 and N3 (P<0.05); 72.5%,94.7% and 60.3%,84.2% in M0 and M1 stages, There were statistically significant differencebetween two stages (P<0.001)3. InⅠ,Ⅱ,Ⅲ,Ⅳclinical stages, the positive expression rates of Ezrin and RhoA proteins in NPC tissue were:50%,40%,80.7%,86.9% and 0%,26.7%,64.9%,80.3%, there were statistically significant difference between every stages (P<0.001)4. The expression of Ezrin and RhoA in NPC were correlated significantly (Rs=0.486, P=0.000)5. The 1-,3-, and 5-year overall survival (OS) rates of the positive expression of Ezrin and RhoA 75%,55%,33% and 84%,60%,33% lower than which negative expression rates 100%,92%,78% and 93%,71%,63%, there were statistically significant (χ2=12.943, P=0.000 andχ2=9.869, P=0.002).Conclusions:1. Ezrin and RhoA protein expression may have relation with the generation of NPC.But the more possibility that is pointing out the positive expression cells maybe have the metastasis potential.2. Ezin and RhoA protein expression was correlated with TNM stages and clinical stages. It suggested that Ezrin and RhoA were key factors in focal metastasis, lymphatic metastasis, organic metastasis.3. Ezrin and RhoA protein expression had a significant correlation and made a further proof that, the two proteins in structure, function and genetic expression had a close connection, meanwhile it showed that the two proteins may be synergic facts in the invasion and metastasis of NPC.4. Ezrin and RhoA were linked with prognosis. The positive expression of Ezrin and RhoA protein in NPC was higher, the survival period will be shorter and prognosis will be worse.. Especially the common positive expression of Ezrin and RhoA protein survival period were obvious shorter than which common negative expression. It will be more significance test Ezrin and RhoA proteins together for predict prognosis.