Study On Pharmacodynamics Of Relieve Fever, Promoting Digestion And Reducing Jaundice Of Folium Microcotis

Folium Microcotis in Guangdong is a conventional native drug, with the effect of relieving dyspepsia and clearing away heat evil and promoting diuresis, can be used for dyspeptic retention, fever caused by exogenous pathogens and jaundice with damp-heat pathogen, has been widely used in clinical. About the study on Pharmacodynamics of Relieve Fever, Promoting Digestion and Reducing Jaundice of Folium Microcotis were no reports on efficacy, the subject selected antipyretic, promoting digestion, reducing enzyme and reducing jaundice, anti-inflammatory, analgesic and other experiments, under the guidance of the theory of Traditional Chinese Medicine, methods and results are as follows:(1) Antipyretic:Subcutaneous injection of 20% of body weight dose of yeast suspension 8ml/kg for 7h, the rat body temperature had rise to maximum, at this time the high dose group and the middle dose group of aqueous extract from Folium Microcotis compared with the control group, there was significant difference(P＜0.05), and the temperature change (△℃) to near normal levels of basal body temperature; In paragraph 5h, blank rat body temperature change (△℃) reduce to a relatively low level, while the low dose and blank group, there were significant differences (P<0.05), and the temperature change (△℃) basal body temperature close to normal levels. The results suggest that aqueous extract Folium Microcotis have better antipyretic effect and can dry yeast-induced changes in body temperature band remained at near normal levels.(2) Promote digestion:With irrigating the black nutritional semi-solid paste, observing the effects of the different extracts from Folium Microcotis on gastric emptying and the propulsive ratio of mice's small intestine; Through the experiment affected by gastric juice secretion in rats, observing the different Folium Microcotis extracts on gastric juice volume, pH, and pepsin activity.①Compared with the control group, Xiangshayangwei pill group, aqueous extract group of Folium Microcotis, ethyl acetate group and surplus water group can significantly reduce the area group residual rate of the mice stomach, small intestine also on a significant role in promoting (P < 0.05 or 0.01).②Compared with the control group, Xiangshayangwei pill group, n-butanol and surplus water group can increase gastric juice volume was significantly (P< 0.05); Xiangshayangwei pill group, ethyl group, n-butyl alcohol group and surplus water group can significantly reduce the gastric juice pH values (P<0.05 or 0.01); Each group, only n-butanol could significantly increase pepsin activity (P<0.01). These results suggest Folium Microcotis can reduce the gastric emptying rate, the promotion of small intestine, increased gastric secretion, decreased gastric acidity and pepsin activity to enhance and promote digestion. The aqueous extract, ethyl acetate extract, n-butanol extract and the remaining parts of the water layer of Folium Microcotis contained the efficacy component parts.(3) Reducing enzyme and reducing jaundice:Usingα-naphthyl isothiocyan-ate (ANIT) induced the mice model of cholestasis liver injury, observing the extracts of Folium Microcotis on total bilirubin(T-BIL) levels, alkaline phosphatase (ALP) activity, aspartate aminotransferase (AST) activity and alanine aminotransferase (ALT) activity in the serum of the mice model.①With the model control group, all groups of Folium Microcotis can significantly reduce the serum levels of T-BIL and D-BIL on model animal, and lower level close to the normal control group, and significantly inhibited ALP, AST and ALT enzyme activity (P＜0.01), that aqueous extract from Folium Microcotis had obvious effect on reducing enzyme and receding jaundice.②The treatment group compare with model group, Yinzhihuang group and surplus water group could significantly reduce the ANIT induced cholestasis model of serum levels of T-BIL, ALP activity, AST activity, ALT activity and liver index (P＜0.05 or 0.01); petroleum group and ethyl acetate group were no statistically significant difference (P＞0.05). Showing that aqueous extract, n-butanol and the part of surplus water layer with significant role on reducing enzyme and reducing jaundice, while other parts, such as petroleum ether, ethyl acetate extract had no reducing the enzyme jaundice effect. These results suggest the active constituents of Folium Microcotis exist in surplus water extract and surplus water extract. (4) Anti-inflammatory effect:Various inflammatory models, including the swelling of ear induced by xylene in mice, the peimeability increase of Capillary permeability by acetic acid in mice were used to explore the anti-inflammatory of Water-extract of Folium Microcotis. The results showed that high and middle doses of Folium Microcotis could significantly inhibit ear edema(P＜0.05 or 0.01), and all the three doses of Folium Microcotis could significantly inhibit the peimeability increase of Capillary permeability (P＜0.05). The results suggest that aqueous extract of Folium Microcotis has significant effect against acute inflammation.(5) The analgesic effect:The mice hot plate and acetic acid induced writhing response observed Folium Microcotis aqueous extract of pain inhibition. The results showed that water extract of Folium Microcotis can each dose group was significantly inhibited in mice due to thermal stimulation caused by pain response (P<0.05 or 0.01); the high and low dose group was significantly inhibited in mice caused by chemical stimulation pain response (P＜0.05 or 0.01). The results suggest that aqueous extract of Folium Microcotis has good analgesic effect.