Research On The Relationship Between H.pylori-Associated Gastropathy Belonging To Splenogastric Hygropyrexia Syndrome And Th1/Th2 Balance

Treatment Based on Syndrome Differentiation is the foundation of Traditional Chinese Medicine (TCM).Splenogastric Hygropyrexia Syndrome is the common one of syndrome in the Splenogastric theory, which is one of important part of TCM. Splenogastric hygropyrexia syndrome originated as a result of the interaction of intrinsic and extirnsic factors, and it belongs to excess syndrome which is a pathological condition--both vital qi and evil qi are strong and struggle with each other. Nowadays splenogastric hygropyrexia syndrome becomes the focus of attention, some researchers made good use of advanced technology and explain every aspect clearly from gastric morphology, gastric motility, gastrointestinal hormones, inflammatory factors and protective factors, immunology, metabolism and microecology.As the most important digestive disease risk factors, helicobacter pylori (Hp) are well known gradually. Research shows that splenogastric hygropyrexia syndrome account for a large proportion in H. pylori-associated gastropathy, and liver-stomach disharmony syndrome and blood stagnant syndrome as the main type of excess syndrome are also important. H. pylori-associated gastropathy particularly at its inflammatory active stage, it's proved that the clinical manifestations and pathological condition are similar to the pathogenic features of splenogastric hygropyrexia syndrome. On the other hand, a great deal of clinical observation and pharmacological studies have shown that some chinese traditional formula and patent medicine treated for splenogastric hygropyrexia syndrome also has a significant effect on the H. pylori eradication. In summary, there is a close relationship between splenogastric hygropyrexia syndrome and H. pylori. Some Chinese scholars take it for granted that H. pylori infection should be the "evil qi" category, H. pylori infection and "dampness and heat" pathogenic factors are equal.After nearly 30 years of research and development, the pathogenetic mechanism of H. pylori can be divided into:colonization in the gastric mucosa, gastric mucosal damage induced by H. pylori, gastric mucosal damage mediated by the host immune response, host condition and some major factors, but there are still many unknown areas need to be further explored. The results show that, H. pylori infection lead to a large number of infiltrating CD4+ T cells as the main types of lymphocytes infiltrating into gastric epithelium and lamina propria. CD4+ T cells represent a kind of cells which have important function to regulate proliferation and immunity of T lymphocytes, so it can also be called as helper T lymphocytes (Th). According to some reseach at present, helper T cells differentiate into Thl and Th2 types. Under normal physiological conditions, the body's Thl and Th2 maintain in a state of equilibrium, and an imbalance of Thl/Th2 may increase the possibilities of suffering from disease. When Thl occupy a dominant position in the balance of Thl/Th2, the immune system eradicate different kinds of pathogens and control the development of major infection, but it tend to lead to severe pathological damage of human body; When Th2 occupy a dominant position in the balance of Thl/Th2, the pathological production and the function of immune system are inhibited, resulting in the establishment of chronic disease development.Studies show that the balance of Thl/Th2 is closely related with many diseases, and there were some debates on the relationship between Thl/Th2 balance and H. pylori-associated gastropathy. Recently, some scholars tend to agree that the effect of protective immunity in H. pylori-associated gastropathy comes from the cooperation of thl and th2 cells. On the other hand, some scholars studied the efficacy of traditional Chinese medicine, and found that different stage of different disease and different treatment methods have shown the differences of Thl/Th2 balance, they think there may exist some regularity and relevant between Thl/Th2 balance and the theory of Traditional Chinese Medicine.Objective:The study is investigating the level of IFN-γ,IL-12,IL-4,IL-10 expression in peripheral blood, in order to analyse the relationship between splenogastric hygropyrexia syndrome in H. pylori-associated grastropathy and Th1/Th2 balance on the whole human body.And also contrast it with three types of splenogastric hygropyrexia syndrome(heavy dampness impairing heat, heavy heat impairing dampness, simultaneous onset of dampness and heat) and splenic deficiency syndrome group. To explore the pathomechanism of the H. pylori-associated gastrosia with splenogastric hygropyrexia syndrome, to illustrate that the different type of syndrome have different pathogenesis, in order to provide the basis for further strengthen the modern research of splenogastric hygropyrexia syndrome, improve the approach to the nature of spleen, and raise the clinical therapeutic effect of traditional Chinese medicine treatment of the spleen and stomach disease.Method:1. We chose 67 cases gastropathy patients including 52cases belonging to splenogastric hygropyrexia syndrome (among them,9 cases belong to the group of heavy heat impairing dampness, and 21 cases belong to the group of simultaneous onset of dampness and heat, and 22 cases belong to the group of heavy dampness impairing heat) and 15 cases splenic deficiency syndrome. And we recruited 12 healthy people as normal control group.2. After disinfection, we got the blood of all objects by the nurse for the preparation of serum.3. H.pylori was detected through ASSURE H. pylori rapid test.4. We detected the expression of IFN-γ,IL-12,IL-4,IL-10 in serum through the liquid chip method.Result:1. Analysis of Common Materials.The difference has no statistical significance on sex, age and the course of disease in every group(P>0.05). So we thought that all of the groups can compared each other.2. Analysis of H. pylori Infection.The rate of H.pylori infection of the group of splenogastric hygropyrexia and splenic deficiency syndrome are significantly higher than normal control group(P≤0.05), but the difference has no statistical significance between splenogastric hygropyrexia syndrome and splenic deficiency syndrome in groups. Besides, in the gastrogathy belonging to splenogastric hygropyrexia syndrome group, the difference has no statistical significance between Hp-positive and Hp-negative on the symptoms including yellow greasy tongue coating, stomach pain or gastric distention, anorexia and loose stool (P>0.05).3. Relationship between the expressions of IFN-γ,IL-12,IL-4,IL-10 in peripheral blood of all groups.The expressions of IFN-γ,IL-12 in splenogastric hygropyrexia syndrome group are higher than that in splenic deficiency syndrome group and normal control group; The expression of IL-4 is lower than that in splenic deficiency syndrome group and normal control group; The expression of IL-10 in all groups is in a low lever or does not exist. The expression of IFN-γ,IL-12,IL-4,IL-10 in splenogastric hygropyrexia syndrome group are all higher than that in splenic deficiency syndrome group. But all the datas have no statistical significance (P>0.05).4. Relationship between the expressions of IFN-γ,IL-12,IL-4,IL-10 in the three types of splenogastric hygropyrexia syndrome group.There is a tendency that the expressions of IFN-γ,IL-12,IL-4,IL-10 in the group of simultaneous onset of dampness and heat are higher than the group of heavy heat impairing dampness, which them are higher than the group of heavy dampness impairing heat, but all the datas have no statistical significance(P>0.05). Besides, there are not the same tendency between IFN-γ/IL-4 and IL-12/IL-4 in these three types of splenogastric hygropyrexia syndrome group.5. Analysis of The Correlation between The Level of H. pylori and The Balance of Thl/Th2.In the group of splenogastric hygropyrexia syndrome, the expression of IFN-γis lower in Hp-positive group than in Hp-negative group, the expression of IL-12 is higher in Hp-positive group than in Hp-negative group, the expressions of IL-4 and IL-10 are all lower in Hp-positive group than in Hp-negative group. But all the datas have no statistical significance(P> 0.05). There is no correlation between the level of H.pylori and The Balance of Thl/Th2.Conclusion:1. There is no significant correlation between H. pylori and the symptoms of gastropathy belonging to splenogastric hygropyrexia syndrome.2. The texture of excess syndrome of splenogastric hygropyrexia syndrome probably reflects the pathological mechanisms when Thl occupy a dominant position in the balance of Thl/Th2, and the balance of Thl/Th2 can probably present the strong or weak condition of vital qi in human body.3. There is a tendency that Thl in the balance of Thl/Th2 take more important role in the group of simultaneous onset of dampness and heat than the group of heavy heat impairing dampness and the group of heavy dampness impairing heat. 4. There is no correlation between the level of H. pylori and The Balance of Thl/Th2.