Study On The Expression Of IL-8 And HSP70 In Hp-related Gastropathy With Splenogastric Hygropyrexia Syndrome

Aim:By detecting the Helicobacter pylori(Hp) infection,interieukin-8(IL-8) and heat shock protein 70(HSP70) expressions of Hp-related chronic superficial gastritis(CSG) and peptic ulcer(PU) patients with splenogastric hygropyrexia syndrome,and contrast it with deficiency of spleen-QI Syndrome, to explore the pathomechanism of the Hp-related gastrosia with splenogastric hygropyrexia syndrome,to illustrate that the different type of syndrome have different pathogenesis,in order to provide the basis for further strengthen the modern research of splenogastric hygropyrexia syndrome,improve the approach to the nature of spleen,and raise the clinical therapeutic effect of traditional Chinese medicine treatment of the spleen and stomach disease.Methods:1.Choose 75 patients with CSG and PU,11 cases belong to the group of splenogastric hygropyrexia syndrome Hp(+) and heavy dampness impairing heat, 10 cases belong to the group of splenogastric hygropyrexia syndrome Hp(+) and simultaneous onset of dampness and heat,11 cases belong to the group of splenogastric hygropyrexia syndrome Hp(+) and heavy heat impairing dampness, 21 cases belong to the group of splenogastric hygropyrexia syndrome Hp(-),12 cases belong to the group of deficiency of spleen-QI syndrome Hp(+),and 10 cases belong to the group of deficiency of spleen-QI syndrome Hp(-).10 cases of recruitment of healthy volunteers as normal control group.Subjects in each group have completed the clinical observation of form,by the person asked to carry out assessment of symptoms score and complete the forms.2.The tunica mucosa sinus ventriculi of all the cases were collected under the gastroscope.The inflammation of mucosa was observed by gastroscope and common hematocylin-eosin staining method.Hp was detected by toluidine blue staining method and fast urease test,two positive results were diagnosed with Hp-positive,two negative results were diagnosed with Hp-negative,one positive and the other negative results were not included in this study.3.The clinical data of all the cases were analyzed.4.The protein expression of HSP70 and IL-8 were detected by immunohistochemistry.5.The mRNA expression of HSP70 and IL-8 were detected by FQ-PCR (Fluorescence Quantitive Polymerase Chain Reaction).Results:1.There was no ignificant difference of the general situation(gender, age)(P＞0.05),comparable in each group.There was no ignificant difference of the distribution of the type of illness(CSG,PU)(P＞0.05),comparable in each group.2.There was no significant difference of the Hp infective rate between splenogastric hygropyrexia syndrome group and deficiency of spleen-QI syndrome group(P＞0.05).Hp infective rate of splenogastric hygropyrexia syndrome group is 60.38%.Hp infective rate of deficiency of spleen-QI syndrome group is 54.55%.The Hp detection rates had the tendency that splenogastric hygropyrexia syndrome group＞deficiency of spleen-QI syndrome group.There was no significant difference of Hp infection level in all the Hp infected groups (P＞0.05).3.There was no significant difference in the main clinical symptom score of the CSG and PU patients with splenogastric hygropyrexia syndrome between Hp-positive group and Hp-negative group(P＞0.05).4.The mucosa inflammation in pathology of splenogastric hygropyrexia syndrome group was more severe than the normal control group(P＜0.05),and in the same type of syndrome Hp-positive group was more severe than the Hp-negative group(P＜0.05).The mucosa inflammation activity in pathology of splenogastric hygropyrexia syndrome group was more severe than the normal control group(P＜0.05),and in splenogastric hygropyrexia syndrome Hp-positive group was more severe than the Hp-negative group(P＜0.05).The Hp gradient of infection and gastric mucosa inflammation was positively correlated(P＜0.001),the correlation coefficient was 0.672;Hp infection and gastric mucosa with acute inflammatory activity was positively correlated(P＜0.001),the correlation coefficient was 0.683. 5.HSP70 protein expression of splenogastric hygropyrexia syndrome group was significantly higher than deficiency of spleen-QI syndrome group and the normal control group(P＜0.05).The expression of splenogastric hygropyrexia syndrome Hp(+) group was significantly higher than splenogastric hygropyrexia syndrome Hp(-) group,deficiency of spleen-Ql syndrome Hp(-) group and normal control group(P＜0.05).The expression of three sub-divided groups of splenogastric hygropyrexia syndrome were significantly higher than normal control group(P＜0.05).The heavy heat impairing dampness group was significantly higher than deficiency of spleen-QI syndrome Hp(+) and Hp(-) group(P＜0.05).The simultaneous onset of dampness and heat group was significantly higher than deficiency of spleen-Ol syndrome Hp(-) group (P＜0.05).The splenogastric hygropyrexia syndrome Hp(-) group was significantly higher than normal control group(P＜0.05).Among the three sub-divided groups of splenogastric hygropyrexia syndrome Hp(+),there was a tendency of HSP70 protein expression:heavy heat impairing dampness＞simultaneous onset of dampness and heat＞heavy dampness impairing heat,but there were no significanct difference among them(P＞0.05).HSP70 mRNA expression of splenogastric hygropyrexia syndrome group was significantly higher than normal control group(P＜0.05).The splenogastric hygropyrexia syndrome Hp(+) group was significantly higher than splenogastric hygropyrexia syndrome Hp(-) group,deficiency of spleen-QI syndrome Hp(-) group and normal control group(P＜0.05).The heavy heat impairing dampness group was significantly higher than splenogastric hygropyrexia syndrome Hp(-) group,deficiency of spleen-QI syndrome Hp(-) group and normal control group (P＜0.05).The simultaneous onset of dampness and heat group was significantly higher than deficiency of spleen-QI syndrome Hp(-) group and normal control group(P＜0.05).The deficiency of spleen-QI syndrome Hp(+) was significantly higher than normal control group(P＜0.05).Among the three sub-divided groups of splenogastric hygropyrexia syndrome Hp(+),there was a tendency of HSP70 mRNA expression:heavy heat impairing dampness＞simultaneous onset of dampness and heat＞heavy dampness impairing heat,but there were no significant difference among them(P＞0.05).6.IL-8 protein expression among all the groups had no significant difference(P＞0.05).There were tendencies of IL-8 protein expression: splenogastric hygropyrexia syndrome group＞deficiency of spleen-QI syndrome group＞normal control group,heavy heat impairing dampness＞simultaneous onset of dampness and heat＞heavy dampness impairing heat,Hp-positive group was higher than Hp-negative group.IL-8 mRNA expression of splenogastric hygropyrexia syndrome group was significantly higher than normal control group(P＜0.05).The splenogastric hygropyrexia syndrome Hp(+) group was significantly higher than splenogastric hygropyrexia syndrome Hp(-) group,deficiency of spleen-QI syndrome Hp(-) group and normal control group(P＜0.05).The heavy heat impairing dampness group was significantly higher than splenogastric hygropyrexia syndrome Hp(-) group,deficiency of spleen-QI syndrome Hp(-) group and normal control group (P＜0.05).The simultaneous onset of dampness and heat group was significantly higher than normal control group(P＜0.05).The heavy dampnes impairing heat group was significantly higher than normal control group(P＜0.05).Conclusion:1.The Hp detection rates of CSG and PU had the tendency that splenogastric hygropyrexia syndrome group＞deficiency of spleen-QI syndrome group.There was no significant difference of Hp infection level in all the Hp infected groups.2.Clinical symptom score and Hp infection of the CSG and PU patients with splenogastric hygropyrexia syndrome did not find significant correlation.Hp infection was not the only factor of splenogastric hygropyrexia syndrome, which also had other influencing factors.3.The degree of the mucosa inflammation and inflammatory activity of CSG and PU patients may be related to the type of syndrome.The inflammation of splenogastric hygropyrexia syndrome group was obviously more severe than the normal control group,and compared with deficiency of spleen-QI syndrome group it had more severe tendency.4.The degree of the mucosa inflammation and inflammatory activity of CSG and PU patients may be related to the Hp infection,Hp-positive group was more severe than the Hp-negative group.The level of Hp infection possibly positive correlated to the degree of the mucosa inflammation and inflammatory activity. The higher level of Hp infecton as while as the more severe mucosa inflammation would exist at the same time.5.The tendencies of HSP70 protein and mRNA expression are splenogastric hygropyrexia syndrome group＞deficiency of spleen-QI syndrome group＞normal control group,and heavy heat impairing dampness＞simultaneous onset of dampness and heat＞heavy dampness impairing heat.HSP70 expression may be prompted with the certificate type of syndrome may exist a certain degree of correlation.HSP70 was obviously expressed in pyretic syndrome.The proportion of heat or dampness in splenogastric hygropyrexia syndrome can influence the degree of HSP70 expression.6.HSP70 protein and mRNA expression of Hp-positive group was higher than that of Hp-negative group in splenogastric hygropyrexia syndrome group while HSP70 protein and mRNA expression of Hp-positive group had the higher tendency than that of Hp-negative group in deficiency of spleen-QI syndrome.With gastric mucosal protective effect of HSP70 may represent a certain extent, part of the body antipathogenic qi.Hp infection may increase the mucosa inflammatory,and to cause and increase the mucosal damaged,it may to some part of the representatives of external pathogenic factors.The relationship between HSP70 expression levels and Hp infection may be partly reflected the "antipathogenic qi fighting with pathogenic factors" state of the plenogastric hygropyrexia syndrome.7.The tendencies of IL-8 protein and mRNA expression are splenogastric hygropyrexia syndrome group＞deficiency of spleen-QI syndrome group＞normal control group,and heavy heat impairing dampness＞simultaneous onset of dampness and heat＞heavy dampness impairing heat.IL-8 is a powerful neutrophil chemotactic factor,and it is related with the inflammatory reaction.IL-8 expression levels may be related to the certain type of syndrome.8.IL-8 protein and mRNA expression of Hp-positive group had the higher tendency than that of Hp-negative group.IL-8 took an important place in the complex cytokine network,can activate and chemotaxis neutrophils,and played an important role in the inflammatory reaction.It is possibly one of the mechanisms that Hp attack on gastric mucosa in splenogastric hygropyrexia syndrome.