The Level Of Anti-sperm Protein 17 Antibodies And Anti-acrosin Antibodies In Human Serum And The Correlation With Infertility

Infertility is an important problem in the research of human reproduction. According to statistics, the incidence of infertility is increasing year by year. Currently, infertility is the inability of a couple to become pregnant after 1 year of unprotected sexual intercourse. The incidence of infertility varies around the world, dying to national, ethnic and regional differences. The incidence rate of infertility is 7%-10%in China, which between 10%-20%of couples experiencing unexplained infertility problems. Infertility is a significant public health problem, which affects both men and women mental and physical health, dying to its etiology and pathogenesis has not been fully clarified. Therefore, affecting the infertility diagnosis, treatment and prevention improve.Sperm as a hidden antigen, contacting with the body's immune system can produce the same kind or autoimmune response, which can produce anti-sperm antibodies. Understanding of the sperm antigens, will help us to diagnosing and treating unexplained infertility, as well as to exploitating contraceptive vaccine. Therefore, the establishment of sensitive, accurate and reliable detection of anti-sperm antibodies in clinical diagnosis and treatment of immune infertility has a wide clinical application.In fact, not all of the anti-sperm antibodies associated with infertility. Only those targeted at the fertilization process of anti-sperm antibodies play a key role to impair fertility. Therefore, the detection of antisperm antibodies of specific target protein antigen is the key to predict infertility.Aim:To detect the role of anti-sperm protein 17 antibodies and anti-acrosin antibodies in patients with infertility. Methods:The levels of AcrAb and Sp17Ab in serum were detected from 24 patients with unexplained infertility (men 12 and women 12),40 patients (men 20 and women 20) with infertility for others reason and 50 normal pregnant people (men 25 and women 25) who have a child in the past years by enzyme linked immunosorbent assay (ELISA).Results:(1) The level of AcrAb in serum of men and women:serum level of AcrAb (4.15±1.18)μg/L, (3.28±1.08)μg/L in unexplained infertility were significant higher than that in control group(1.49±0.51)μg/L, (1.50±0.48)μg/L and infertility with other reason (1.56±0.40)μg/L, (1.50±0.44)μg/L, (P<0.01). The control group and infertility with other reasons have no significant differences (P>0.05). (2) The level of Sp17Ab in serum of men and women:serum level of Sp17Ab in unexplained infertility (8.34±1.88)μg/L, (7.89±2.22)μg/L were significant higher than that in control group (4.17±1.07)μg/L, (3.54±0.63)μg/L and infertility with other reason (4.10±0.95)μg/L, (3.58±0.93)μg/L, (P<0.01). The control group and infertility with other reasons have no significant differences (P>0.05). (3) The level of AcrAb in serum of men and women:serum level of AcrAb (4.15±1.18)μg/L of men and women (3.28±1.08)μg/L in unexplained infertility have no significant differences (P>0.05). (4)The level of Sp17Ab in serum of men and women:serum level of Sp17Ab of men (8.34±1.88)μg/L have no significant differences than women (7.89±2.22)μg/L with unexplained infertility(P>0.05). (5) The level of AcrAb and Sp17Ab in serum of men and women have no significant relationship(r=0.125,P>0.05, r=0.565, P>0.05).(6) The level of AcrAb in serum of men and women with unexplained infertility have positive correlation (r= 0.624, P<0.05).But the level of Sp17Ab in serum of men and women with unexplained infertility have no correlation (r=-0.302, P> 0.05).Conlusion:1. The level of AcrAb and Sp17Ab in serum of men and women with unexplained infertility were significantly higher than that in control group and infertility with other reason, suggesting that AcrAb and Sp17Ab were significantly correlated to infertility.2. The level of AcrAb and Sp17Ab in serum of men and women with unexplained infertility have no significant relationship(r= 0.125, P>0.05, r =0.565, P>0.05), This shows detection of AcrAb can not replace the Sp17Ab.3. The level of AcrAb in serum of men and women with unexplained infertility have positive significant relationship (r= 0.624, P<0.05). The level of Sp17Ab in serum of men and women with unexplained infertility have no significant relationship(r=-0.302, P>0.05). We proposed that the level of AcrAb in serum of men with unexplained infertility increased, making their spouses more easily produce AcrAb. Moreover, The level of Sp17Ab in serum of men with unexplained infertility arising didn't suggest that their wives have Sp17Ab, because we can detect Sp17 expression in healthy male and female reproductive tract, trachea, throat and Ciliated epithelial cells of lungs.