| Objective:To assess the variation of main clinical indicators of Hepatocellular carcinoma (HCC) as well as changes in the genome of microsatellite DNA associated with Hepatocellular carcinoma (HCC).Methods:A total of 30 Hepatocellular carcinoma (HCC) inpatients were included in the Hepatocellular carcinoma (HCC) group,15 males and 15 females, aged from 37 to 81 years old, the average age of 62.17 years. The biochemical control group recruited 30 healthy individuals, aged between 40 and 80 years old, the average age of 60.53 years,who were randomly selected from the physical examinees. The STR control group consisted of 100 healthy individuals without HCC,including 50 males and 50 females, which matched HCC group. Venous blood was collected for the detection of total protein (TP), albumin (ALB) to estimate the liver function,for the detection of triglyceride (TG), high density lipoprotein (HDL),low density lipoprotein (LDL) and glucose (GLU),for the detection of creatinine (CREA) and urea (UREA) for evaluating the renal function,for the detection of immunization items IgA, IgM, IgG, plus a super-sensitive CRP (h-CRP).All the above detections were carried out with the Hitachi 7600 Fully Automatic Biochemical Analyzer. The measure of WBC, Neu#, LY#, RBC, HGB, PLT was performed by the sysmex XE2000 automated analyzer. Tumor markers, included AFP, CEA, CA153, NSE, CA50,were conducted by means of chemiluminescence methods. And STR locis were carried with a polymerase chain reaction-restriction assay and the five-color fluorescence automatic detection technique,including D8S1179,D21 S1 1, D7S820,CSF1PO,D3S1358,D5S818,D13S317,D16S539,D2S1338,D19S433, VWA,D12S391,D18S51,D6S1043,FGA.Results:The level of liver function items TP (g/L), ALB (g/L) on average in the Hepatocellular carcinoma (HCC) group were respectively 69.11 and 38.92,while in the control group they were respectively 73.41 and 45.71. There was a significant difference in the two items (P<0.05). Renal item UREA (mmol/L)in the Hepatocellular carcinoma (HCC) group averaged 7.4, while 4.77 in the control group. Again there was a significant difference in the test (P<0.05). However,the other test CREA (μmol/L) has no significant difference(P>0.05). The average values of blood components RBC,HGB,Neu# and LY# in the Hepatocellular carcinoma (HCC) group were respectively 4.01,118.53,4.83,1.45. while in the control group such average values were 4.47,129.90,3.09 and 2.12. There was a significant difference in these four items (P<0.05), while there was no significant difference in the other items. Comparing the HCC group with the control group,the level on average of blood glucose(GLU, mmol/L) and lipids:high density cholesterol (HDLC, mmol/L) was 1.17 versus 1.62, there was a significant difference in this item (P<0.05), but there was no significant difference in blood glucose(GLU, mmol/L), triglyceride (TG mmol/L) and low density cholesterol (LDLC, mmol/L) (P>0.05). In the ultra-sensitive CRP (h-CRP) test,the result of h-CRP had a significant difference (P>0.05). Comparing IgG(mg/L) in the Hepatocellular carcinoma (HCC) and in the control group(P<0.05),there was a significant difference, but in IgM(mg/L) and IgA(mg/L) items,there were no significant difference. The tumor marker AFP(ng/ml),CEA(ng/ml) in the Hepato-cellular carci-noma (HCC) and the control group had significant difference (P<0.05), there was no significant difference in the other markers.The loci frequencies of STR D8S1179-14,D19S433-14,D6S1043-20 in the Hepato-cellular carcinoma (HCC) group were respectively 0.300,0.450,0.117,and the same frequencies in the control group were respectively 0.170,0.270,0.030, there was significant difference in these three locis (P<0.05) while there was no significant difference in the other locis.Conclusion:Changes in liver function, renal function, regular blood lipids,tumor markers and immune system occur to different degrees in the Hepatocellular carcinoma (HCC) patients. The loci frequencies of STRs D8S1179-14,D19S433-14,D6S1043-20 are higher than those in the control group, so there may be HCC susceptibility genes nearby.
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