The Effects Of Alarelin On Nude Mice Bearing Human Endometrial Carcinoma And The Expression Of Pten

ObjectiveRecently, epidemiological studies have found that the application of gonadotropin releasing hormone analogues(GnRH-a) is related to the degression of morbidity and mortality of many hormone-dependent tumors. But most studies have focused on the aspect of endometriosis, uterinemyoma, ovarian carcinoma,breast carcinoma and prostate,carcinoma etc.Compared with few about endometrial cancer.In this study, To study if Alarelin can inhibit nude mice bearing human endometrial carcinoma and to explore its molecular mechanisms.Materials and MethodsHuman endometrial carcinoma HEC-1-B cells were cultured in vitra.They were inoculated to 24 athymic mice at the right lower extremity near the fold inguen. Then the mice were randomly divided into blank control group, low-dose group, middle-dose group and high dose group.After 3 days of the inoculation,we injected subcutaneouly the mice of low-dose group (15ug/kg), middle-dose group(30ug/kg) and high dose group(60ug/kg) with Alarelin one time every day for 6 weeks and the blank control group were injected with the isodose isotonic Na chloride in the same way.During the period of injection with Alarelin, we measured the tumor size and observed the condition of the mice.At the end of the experimentation,the tumors were dislodged completely then we measured the weight of tumors,drawed the growth curve of the tumors, calculated the inhibition rate of the tumor and detected the expression the Pten with the method of RT-PCR.Results1.The growth of tumor was inhibited when the lowest Alarelin concentration 15ug /kg was given, inhibition rate of the tumor was 16.70%. The inhibition rate of the tumor increased with the rising of Alarelin concentrations. When Alarelin concentration was 60ug/kg, the inhibitory rate increased to 31.30%, the difference was significant (P <0.05).2.After 6w with 15ug/kg to 60ug/kg Alarelin, the level of Pten increased. The difference was significant (P< 0.05)Conclusion1.Alarelin might have directed inhibitory response on nude mice bearing human endometrial carcinoma.2.Up-regulation of Pten protein may be one of the mechanisms through which Alarelin induced growth inhibition of nude mice bearing human endometrial carcinoman.