Expression And Significance Of TAMs And EP3 In Human Ameloblastomas

ObjectiveThis study was to investigate the influence of Tumor-associated macrophages (TAMs) infiltration in the human ameloblastomas(ABs), the correlation between TAMs and angiogenesis in ABs, to investigate the expression and significance of prostaglandin E2(PGE2) receptor EP3 in the ABs.Materials and MethdodsAll of the samples were selected from department of oral pathology, school of stomotology, China Medical University in the period of 2001 to 2007. Include: ameloblastomas (n=101, including 66 primary ABs,35 recurring ABs), normal oral mucosa(n=26).Using the HE convention dyeing, Under microscope observes the neutral granular cell, the macrophage,the lymphocyte, acidophilia granular cell, cytoplasmic cell in human ABs. TAMs, MCD and EP3 were detected by streptavidin peroxidase method and avidin-biotin peroxidose complex technique of immunohistochemistry using monoclonal mouse anti-human CD68 and CD34 antibody, polyclonal rabbit anti-human EP3 antibody.Results1. Observes the ABs clinical pathological section using the HE convention dyeing, discovers in person ABs the macrophage, the lymphocyte, the neutral granular cell obvious infiltration, has the few cytoplasmic cells's infiltration expression, these inflammatory cell some infiltrations in near tumor mesenchymal, some direct infiltrations in tumor epidermis group. Compare the infiltration with the normal mucous membrane to be remarkable, the P value respectively is 0.000, has statistics significance.2. The mean macrophage counts in ABs was significantly higher than the counts in normal mucosa (P<0.05). The mean macrophage counts in primary ABs, recurring ABs were different. The mean microvessel counts in ABs were higher than that in normal mucosa (P<0.05). The mean microvessel were strongly correlated with TAMs counts in ABs (P<0.01).3. EP3 was expressed in the plasma of peripheral columnar or cuboidal cells and central polyhedral cells of odontogenic epithelium, most of which were moderately positive and strongly positive. The expressions of EP3 had heterogenicity.It was strong positive in well differentiated keratinocytes, while in primary embryonic cells, It was negative or weakly positive..EP3 protein expressed strongly in the nuclus of ABs, and its nuclear positive rate was 60.38%(64/106).The expressions of EP3 in the plasm of phlogotic cells, vascular endothelial cells and fibrocytes were also positive. In normal mucosas, EP3 was expressed in surface layer and the superficial layer of stratum spinosum. The immunohistochemical staining of EP3 was obviously expressed in human primary ABs, recurrent ABs. the positive rate was recurrent ABs (85.71%), primary Abs (80.30%).And EP3 also expressed in NOMs, the positive rate of which was 42.31%. Using Rank sum test, we found that there were significant differences between NOMs and Abs (P=0.000).Conclusions1. In human ABs the macrophage, the lymphocyte, the neutral granular cell obvious infiltration, has the few cytoplasmic cell's infiltration expression. The inflammatory cell constituted the ABs tumor microenvironment.2. The expression of TAMs in ABs was stronger than in normal mucosas, and it may play a major role in the regulation of angiogenesis in ABs.3. The protein expressions of EP3 in ABs was higner than that of normal mucosas.