C57BL Mice With Lewis Lung Cancer Effect Of Resveratrol On The Radiation Sensitizing Effect

PurposeAnimal tumor model using C57BL mice with Lewis lung cancer effect of resveratrol on the radiation sensitizing effect.MethodAnimal and tumor model:C57BL/6 inbred mice and Lewis lung cancer model purchased from Chinese Academy of Sciences Cancer Hospital, mice 8-9 weeks old, weight 18-24g, male. Conventional Methods Lewis lung tumor cells removed from the tumor-bearing mice, tumor cell suspension is made,0.2ml (containing cells about 2* 106)/only, mice were inoculated subcutaneously in the right lateral thigh, to be When the average tumor volume reached 0.6cm3 began randomized experiment. Resveratrol and dimethyl sulfoxide were purchased from Sigma company. Will be made of resveratrol dissolved in DMSO mixture. Administration volume for each mouse 5mg/Kg weight 2 hours before radiotherapy single intraperitoneal injection. Will be transplanted with Lewis lung cancer in C57BL mice were randomly divided into 4 groups:(1) control group:no treatment; (2) Radiation alone group:were given 5Gy, 10Gy,15Gy,20Gy dose of radiation therapy; (3) drug group simple:only weight given to each mouse by intraperitoneal injection of drug 5mg/Kg; (4) medication+ radiotherapy group:the weight given to each mouse by intraperitoneal injection of drug 5mg/Kg,2 hours later respectively 5Gy, 10Gy,15Gy,20Gy dose radiation. Radiation after every 1, by hand with a vernier caliper measurements of tumor size, tumor volume. And the survival time of mice was calculated radiosensitivity and tumor growth delay than the time. Results1. When the tumor volume reached 4 times the volume beginning of the experiment, respectively, calculated resveratrol radiosensitization ratio 1.50,1.52, 1.49, an average of 1.50.2. Blank control group and the treatment group the average survival days and to 25.5±3.1d 26.4±4.5d, (t=2.793, P=0.117).3. Blank control group and the drug group were similar to tumor growth of tumors, no significant difference between the groups (t=3.137, P=0.106).ConclusionsC57BL mice with Lewis lung cancer effect of resveratrol on a radiosensitizing effect, and no toxicity in mice.DiscussionResveratrol formula is C14H12O3, is a non-flavonoid polyphenols. Grape and common medicinal plants, such as Cassia, Polygonum can be extracted. Resveratrol not only has anti-inflammatory, antithrombotic, antioxidant, anti-tumor function, and can lead to cell cycle G2/M phase arrest. As in the G2/M phase of the sensitivity of tumor cells to radiation than other cell cycle high, so inference has radiosensitizing effect of resveratrol. The laboratory has rows of resveratrol on human nasopharyngeal carcinoma cells in vitro radiosensitizing effects, the results confirmed that a radiosensitizing effect of radiosensitization ratio of 1.655. Radiosensitization in vitro experiments, but often there is a big bias, is often necessary before animal experiments to clinical trials over. In this study, animal tumor models confirmed the effect of resveratrol on C57BL mice with Lewis lung cancer radiosensitization effects and toxic side effects in mice. But on resveratrol induced cell cycle G2/M arrest in, still need further study and discussion. This can be considered a promising new generation of Chinese medicine resveratrol radiosensitizer, but due to a shorter half-life of resveratrol, nature is not yet stable, but also to further explore and improve the new formulation of resveratrol can be widely application to the treatment of radiosensitization to.