Study The Interaction Between The Active Components Of The Traditional Chinese Medicines Used For Diabetes Therapy And Bovine Serum Albumin

As a very common chronic metabolic disease, diabetes is becoming the third "killer" to the health of mankind along with cancer, cardiovascular and cerebrovascular diseases because of its high prevalence, morbidity and mortality. In our country, there are a lot of Chinese traditional medicine used to cure or alleviate diabetes and its complications. Up to now, it is bewildering the mechnism of these Chinese medicines affecting the disease and there are too much unclear on how and why to use these Chinese medicines.In this paper, we chose Panax notoginseng (Burk.) F. H. Chen as the studied object. The following works were carried out:1.Total saponins of Panax notoginseng were separated into different polar groups by silica gel column chromatography. The interaction between these substances and BSA were studied using fluorescence and which substance(s) affected the protein be found. It would provide scientific and experimental evidence for confirming the effective constituents of Panax notoginseng.2.The interactions of the single ingredient (Ginsenoside Rb1, Rg1 and Re) and the multi-ingredients (Rb1 plus Rg1,Rb1 plus Re, Rg1 plus Re) with BSA at physiological pH were investigated by fluorescence spectroscopy. It provides a molecular basis for elucidating the mechanism of each drug acting alone and its combinations and determining whether or not a given drug combination would gain a synergistic effect.3.Equilibrium dialysis, ultrafiltration combined with high-performance liquid chromatography, and gel-filtration chromatography were used to determine the binding ratio of mangiferin to BSA. The aim of this study was to find simple and rapid methods used for isolating active components of Chinese medicinal plants.This dissertation consists of five chapters:Chapter 1:Diabetes and the traditional Chinese medicines used for diabetes therapy were briefly described. The studied methods of drug and protein binding were reviewed.Chapter 2:Total saponins of Panax notoginseng were separated into four different polar groups by silica gel column chromatography. The interaction between these substances and BSA were studied using fluorescence and which substance(s) affected the protein be found.Chapter 3:The interactions of the single ingredient (Ginsenoside Rb1,Rg1 and Re) and the multi-ingredients (Rb1 plus Rg1,Rb1 plus Re, Rg1 plus Re) with BSA at physiological pH were investigated by fluorescence spectroscopy. It can provide a molecular basis for elucidating the mechanism of each drug acting alone and its combinations and determining whether or not a given drug combination would gain a synergistic effect.Chapter 4:Equilibrium dialysis, ultrafiltration combined with high-performance liquid chromatography, and gel-filtration chromatography were used to determine the binding ratio of mangiferin to BSA. Compared the results get from different methods, gel-filtration is unsuitable for the aim.Chapter 5:Polystyrene resin used as the matrix resin was modified and grafted with the BSA surface, and its adsorption ability of small molecule drugs as well as the possibility of column packing were studied.