| Objective:Cerebral ischemia after a certain time to restore the blood supply, not only failed to restore its function, but appeared more severe brain dysfunction, known as cerebral ischemia-reperfusion injury. Cerebral ischemia-reperfusion injury and free radicals and lipid peroxidation generation, intracellular calcium overload, excitatory amino acid toxicity, high white blood cell aggregation and the lack of high-energy phosphate compounds, gene activation, heat shock protein expression and other related disorders. Recent studies found that cerebral ischemia-reperfusion injury including acute necrosis and DND (delayed neuronal death) of apoptosis. Caused by cerebral ischemia-reperfusion of brain tissue damage, in order to promote their recovery has become the treatment of stroke disease is an important research topic. In recent years, many studies confirm that traditional Chinese medicine on cerebral ischemia-reperfusion injury has a protective effect. This experiment is a study of DXTLD that can protect brain tissue of cerebral ischemia reperfusion injury.According to several years clinical experiences, Professor LiuYun has formed a basic experienced prescription Danxing tongluo decoction (DXTLD). It is a mixture of some herbs, such as Danshen, Dannanxing, Chuanxiong, etc.It has functions of cleaning away stagnated blood and heat, restoring normal functioning of the body to consolidate the constitution and resolving mass. Danshen, Dannanxing are the chief herbs of the dose,which can promote blood flow and deoppilate meridian. Chanxing, Sanqi, Honghua, Dahuang, Huangqi, and Danggui, are the secondary herbs of DXTLD.Huanqin, Huaihua, Yuanshen, and Gancao, are the third herbs. So the dose can efficaciously preclude the sputum stagnated blood and sputum blockaging in brain meridian. On account of appearance and substance are both to be cured, we can get a fine therapeutic effect by using ti to cure the strokeSome research results discover that DXTLD has to improve the efficacy of ischemic stroke symptoms and has a protective effect for cerebral ischemia-reperfusion, but its mechanism has not yet completely clear. This experiment is through the observation of TGF-β1 and IGF-1 in rat cerebral ischemia-reperfusion tissue expression for the clinical application of DXTLD may provide some objective evidence of stroke.Methods:48 healthy SD rats which ignore gender were assigned randomly to 6 groups,8 rats per group:black group, ischemia/reperfusion (I/R) group, DXTLD low dosage group, DXTLD middle dosage group DXTLD high dosage group and Nimodipine(NIMO) group. DXTLD groups were given to DXTLD at dose of 0.75ml/100g,1.15ml/100g 1.5ml/100g,NIMO group at 2ml/100g,once a day. Saline was given to I/R group at 1.Oml/100g weight.and black group without treatment.After 7-days,the rats of all the groups except black group were made into cerebral ischemia reperfusion injury models by thread embolism method. All the rats were killed and their brains were taken out after 2h ischemia and 24h reperfusion injury. The TGF-β1 expression and IGF-1 expression in brain tissue were measured with immune-ohistochemical method.Results:1.The ultrathin sections of brain tissue is made research about histomorphology by the common HE tacho-staining technique after rat cerebral ischemia and reperfusion:The group of black group has normal cellular structure; The margin of focus of infection comparatively is distinct and the degree of cellular necrosis is more serious in the group of I/R group. The cells are obviously cutaneous dropsy. At the center of ischemic area we can find cellula nervosa necrosis, circum-cell diastem become broader, nucleus shrinkages, caryotheca fragments, chromato-spherite disappears, etc; While in the group of DXTLD high medium small group dosage group and NIMO group we can get the outcome that neuronal degeneration fairly light at ischemic region. Besides these, cells comparatively regular and full, and there are integrate living cells at the around of semidarkness area.2. To observe the expression level of TGF-β1 and IGF-1 by the ultrathin sections of brain tissue and the method of immunohistochemistry technology:After the six groups rats had the medicine or substitute medicine and then disparity has significantly disparity between DXTLD high and medium-dose group and I/R group (P<0.05). Videlicet:DXTLD could obviously increase the TGF-β1 and IGF-1 expression in cerebral cortex and hippocampus and relieve nerve cell injury.Conclusions:DXTLD can protect brain tissue of cerebral ischemia reperfusion injury and improve the nerve function. The mechanism may be related with the increasing of TGF-β1 expression and IGF-1 expression.
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