| Objective:Ischemic cerebrovascular disease is a clinical common disease, Frequently-occurring disease, which has a high morbidity and high mortality characteristics, is one of the clinical focus of disease prevention and control. Early cerebral ischemia, cerebral autoregulation can be got to compensatory blood flow, cerebral ischemia when capacity exceeds its compensatory damage arises ischemic. In the early resumption of blood flow within the time window is conducive to reducing infarct size, reduce nerve damage. Early thrombolytic treatment of acute ischemic stroke is the most effective, but because of thrombolytic time window (≤6 hours), only a small number of patients benefit from thrombolysis. By thrombolysis or collateral circulation to ischemic cerebral blood flow recovery, but will increase the original ischemic injury, reperfusion injury produced, causing intracranial hemorrhage, cerebral edema and other serious complications, limits the thrombolytic therapy promoting the use of. How to maximize the reduction caused by cerebral ischemia and reperfusion damage, protect nerve cells, improve survival, reduce the sequelae of a stroke research focus. Ischemic cerebrovascular disease medicine is called "stroke" is common and frequently occurring diseases in the elderly, acute onset, most physicians believe that ischemic stroke in this for the virtual, virtual is qi or liver and kidney yin true, usually qi and blood deficiency, heart, liver, kidney three dirty yin and yang imbalance, blood against the chaos caused by the meridian closed block. Is a disease of the liver and kidney, wind, fire, phlegm, blood stasis is a disease of the standard, so the standard for the real. Stroke mortality was higher after the clinical treatment of dementia will inevitably left behind, hemiplegia, incon- tinence, affective disorder or other consequences, to patients and families of the pressure. Therefore, we applied for the cause of Danxingtongluo decoction (DXTLD) by Tongfu Huayu Tang, expectorant enlighten, Fu Qi and, phlegm to go, Jiuqiao profit, the prevention and treatment of this disease. DXTLD is my years of clinical experience in prescription departments, previous study indicated that Mindan Star Decoction for the symptoms of ischemic stroke has improved the efficacy of Salvia Huoxuetongluo which can effectively remove the phlegm and blood stasis brain contact, Rhubarb, Chuanxiong, Sophora japonica L., March 7, safflower Tongfuxiere, Qi and activating blood, skullcap, Yuan Participation heat phlegm dampness. Various drugs were played Tongfu stasis, expectorant enlighten the effect.Research has shown that, after focal cerebral ischemia leukocyte infiltration to the ischemic area, tissue inflammation is an important mechanism causing brain damage, while the adhesion between the molecule-1 (intercellular adhesion molecule 1, ICAM-1) expression increased, leading to leukocyte infiltration to the main ischemic. Another study found that after cerebral ischemia can induce insulin-like growth factor-1 (Insulin2 like growth factor-1, IGF-1) expression of endogenous and exogenous IGF-1 inhibits apoptosis are protected The role of brain. The experiment by observing the ICAM-1 and IGF-1 in rats with ischemia-reperfusion tissue ex-pression, order to provide evidence for clinical work.Methods:48 healthy SD rats which ignore gender were assigned randomly to 6 groups,8 rats per group:blank group, ischemia/reperfusion (I/R) group,DXTLD low dosage group,DXTLD midst dosage group,DXTLD high dosage group and Nimodipine (NIMO) group. DXTLD groups were given to DXTLD at dose of 0.75ml/100g,1.15ml/100g,1.5ml/100g;NIMO group at 2ml/100g,once a day. Saline was given to I/R group at 1.0ml/100g weight.and black group was nothing.After 7-days,the rats of all the groups except black group were made into cerebral ischemia reperfusion injury models by thread embolism method. All the rats were killed and their brains were taken out after 2h ischemia and 24h reperfusion injury. The ICAM-1 expression and IGF-1 expression in brain tissue were measured with immune- ohistochemical method.Results:1.The ultrathin sections of brain tissue is made research about histomorphology by the common HE tacho-staining technique after rat cerebral ischemia and reper-fusion:The group of black group has normal cellular structure; The margin of focus of infection comparatively is distinct and the degree of cellular necrosis is more serious in the group of I/R group. The cells are obviously cutaneous dropsy. At the center of ischemic area we can find cellula nervosa necrosis, circum-cell diastem become broader, nucleus shrinkages, caryotheca fragments, chromato-spherite disappears, etc; While in the group of DXTLD high dosage group and NIMO group we can get the outcome that neuronal degeneration fairly light at ischemic region. Besides, cells comparatively regular and full, and there are integrate living cells at the around of semidarkness area.2. To observe the expression level of ICAM-1 and IGF-1 by the ultrathin sections of brain tissue and the method of immuno histochemistry technology:After the six groups rats had the medicine or substitute medicine and then disparity has significantly disparity between DXTLD high dosage group and I/R group (P<0.05). Videlicet:Danxingtongluo decoction on cerebral ischemia-reperfusion injury in the expression of ICAM-1 down effect on the expression of IGF-1 upregulate.Conclusions:Danxingtongluo decoction on cerebral ischemia-reper-fusion injury has a protective effect, can improve neurological deficits, and its mechanism may be related to inhibition of ICAM-1 protein synthesis, increase IGF-1 expression.
|
PDF Full Text Request