| Objective: Cerebrovascular disease is threatening human health, the three of today's one of the reasons. One of ischemic cerebrovascular disease and the incidence of hemorrhagic cerebrovascular disease ratio of about 3:1, so ischemic cerebrovascular disease has become the focus of prevention and treatment of cerebrovascular disease. Treatment of acute ischemic cerebrovascular disease is the most effective early thrombolysis, but due to the short time window of thrombolytic therapy (≤6 hours), only a small number of patients benefit from thrombolysis. Or collateral circulation through methods such thrombolysis to restore blood flow to the brain ischemic area, but will increase the original ischemic injury, resulting in reperfusion injury, caused by intracranial hemorrhage, cerebral edema and other serious complications, limit the Extensive use of thrombolytic therapy. How to reduce ischemia reperfusion injury, protect nerve cells, reducing the incidence of sequelae of stroke and improve the survival rate of patients become a research hotspot.Chinese medicine called ischemic cerebrovascular disease of ischemic stroke, The disease is common and frequently-occurring disease in the elderly, with a rapid onset, high morbidity and mortality is high.Many physicians believe that ischemic stroke is the virtual real, Virtual is a weak liver and kidney or blood, liver and kidney in the next, liver-yang hyperactivity in the last, be added daily diet properly, or feel the evils of emotional stimuli, resulting in red blood on the brain, blocking off God awakened .Is a disease of the liver and kidney, wind, fire, phlegm, blood stasis is a disease of the standard, it is marked as a reality.For this cause we apply Danxingtongluo soup through Tongfu Xiere, expectorant resuscitation, blood collateral method, so that bowel gas pass, phlegm to go, Jiuqiao benefits, treatment and prevention of this disease. Danxingtongluo soup is my years of clinical experience department side, arguably the gall Southern Star, skullcap, Scrophulariaceae heat phlegm, Salvia, Chuanxiong, notoginseng, safflower Huoxuetongluo, can effectively remove the phlegm and blood stasis brain network Blood, Rhubarb, Scutellaria Tongfu Xiere. Tongfu various drugs played a total of stasis, expectorant effect of resuscitation.Experimental results demonstrate, Cerebral ischemia in inflammatory response leads to the aggravation of ischemic neuronal injury is the pathological process of cerebral ischemia in a very important part. Inflammatory cells and cytokines, represented by the inflammatory mediators TNF-αinvolved in the regulation of inflammatory response, leading to nerve cell ischemia to cell death from early irreversible damage to the evolution of increased brain damage. In recent years, studies have found that nuclear factor-kB (NF-κB) activation and ischemic neuronal damage are closely related.How to regulate NF-κB activation and expression of inflammatory cytokines is a potential neuroprotective research goals. In this study, TNF-αand by observing the NF-κB in rat cerebral ischemia-reperfusion tissue expression, aimed at clinical application Danxingtongluo provide an objective basis for soup.Method: 40 SD rats of either gender, were randomly divided into sham operation group, ischemia reperfusion group, nimodipine group, Danxingtongluo soup low dose group, Danxingtongluo soup high dose group, n = 8 rats. Sham group and ischemia reperfusion group were fed to 1.0ml/100g saline gavage once daily for 7 days; Nimodipine nimodipine orally once daily (1.0ml/100g), for 7 days; Danxingtongluo soup low dose group (0.75ml/100g), high-dose group (1.5ml/100g) Daily Danxingtongluo Infusion time, for 7 days, 2h on day 7 after surgery drug. With middle cerebral artery suture method produced focal cerebral ischemia reperfusion model (no treatment sham group). Focal cerebral ischemia 2h, and then reperfusion, reperfusion was observed after 24h, using Longa score, those who pass the test TNF-αand NF-κB positive cells expression.Results:1. Using rapid routine HE staining of rat brain after cerebral ischemia ultrathin sections for histological examination: Sham group structure of the normal brain tissue, hippocampal neurons were round or oval, the larger cell body, chromatin distribution, prominent nucleoli. Ischemia-reperfusion rat cortex and hippocampus pyramidal layer of nerve cells in the gap widened significantly in hippocampus neuron degeneration, cytoplasm red, nuclear ambiguity, nuclear condensation or disappeared, increased number of glial cells . The treatment group also can be seen in hippocampus neuronal degeneration, nuclear ambiguity, nuclear condensation or disappear, but the number was significantly less than ischemia reperfusion group, a dose-dependent, high-dose group and the nimodipine group improved the most obvious.2.Detected by immunohistochemistry in brain tissue TNF-α, NF-κB positive cells, The results show that cerebral ischemia-reperfusion group, TNF-αand NF-kB was significantly increased (P<0.01), Danxingtongluo soup large and small dose group TNF-αand NF-κB expression was significantly lower (P<0.01). Tip Danxingtongluo decoction on cerebral ischemia reperfusion injury is closely related with the inhibition of inflammatory response.Conclusion: Danxingtongluo decoction on cerebral ischemia reperfusion injury has obvious protective effect Danxingtongluo soup neuroprotection mechanism may reduce the cytokine-mediated inflammatory reaction. |
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