The Clinic Studies On Detecting The Expression Of SALL4 And BM I-1 MRNA In Leukemia By Real-time Quantitative Reverse Transcription PCR

Objective:To investigate the expression of SALL4 and BMI-1 mRNA in different kinds of leukemia patients through testing the expression of SALL4 and BMI-1 mRNA in acute and chronic leukemia patients and seek a mew method of monitoring minimal residual disease (MRD), conduce to judge curative effect pretest relapse,guide treatment.Methods:In this experiment, real-time quantitative reverse transcription PCR (RT-PCR) was used for detecting the expression of SALL4 and BM I-1 mRNA in the mononuclear cells of 60 leukemia patients and 10 normal controls.Results:The expression of SALL4 n.RNA BMI-1 mRNA in de novo leukemia patients and relapsed patients was higher than that in controls, which significantly decreased at complete remission (CR). The difference between CR group and de novo group and relapsed group was statistically significant (p<0.05). In relapsed patients, the expression of SALL4 mRNA increased, slightly higher than that in de novo leukemia group, but the difference between CR group and de novo group and relapsed group was not statistically significant (p>0.05). The expression pattern of BMI-1 was the same to that of SALL4 except the up-regulation in M3,CLL,M4, and the expression of BMI-1 was positively correlated with that of SALL4 in leukemia (r=0.825,p<0.01).Conclusion:Testing the expression of SALL4 and BMI-1 mRNA in acute and chronic leukemia patients make sense for monitoring minimal residual disease..