The Effects Of Egb Extract On High-intensity Exercise In Mice Antioxidant Capacity And Immune Function

Objectives:With the development of technology, EGB more be applied to human health and the medical field. Its special chemical constituents and their characteristic of the removal of radicals more attention, and improve the antioxidant capacity, to maintain normal immune functions, to increase the power of important measures. Through in animal experiments, by trying to EGB in the intensity of the mouse movement, antioxidant and immune functions, to EGB in improving the strength of the movement of training the body of the antioxidant punch down and suppress immune from the use of reference, and leaves the depth of the genus extract offer a theoretical basis for development and utilization.Method: Let 32 mice that 8weeks old C57BL / 6 randomly divided into 4 groups: sedentary control group 6 (C), simple perfusion group 8 (G), 8 high intensity exercise group 8 (HS), high intensity exercise perfusion group 10 (HS + G). Quiet control group neither perfusion nor movement, simple perfusion group were given by intragastric dose of ginkgo biloba extract, high-intensity exercise group daily high-intensity exercise training, high intensity exercise groups both large-perfusion intensity exercise training has given and the perfusion group the same dose of EGB. All mice were housed separately in standard solid state mixing free diet. After 8 weeks, serum levels of extraction, -20℃preservation.Detection of MDA, SOD, T-AOC and other indicators. At the same time open the peritoneal, spleen organ for spleen lymphocyte suspension was prepared, RT-PCR detection of IL-2 , apoptosis of lymphocytes by flow cytometry and IL-10 .Results:1. MDA:Compared with the C, HS mice MDA content increased, there was a significant difference (P<0.01); HS + G group were slightly higher MDA content, the difference was not significant (P>0.05); G MDA content was decreased in mice, the difference was not significant (P>0.05); and compared HS, HS + G group MDA content increased by less significant difference, (P <0.05). Compared with HS, HS + G MDA content decreased in mice, significant differences (P<0.01).2. T-AOC and SOD:Compared with the C group, HS group were significantly lower SOD mice had significant differences (P<0.05); group HS + G SOD content increased in mice, was significantly higher (P<0.05); group G SOD mice was significantly increased, difference was significant (P<0.01); and compared HS, group HS + G increased SOD has a very significant difference, (P<0.01). Compared with HS, HS + G content increased SOD mice, significant differences (P<0.01).Compared with the C group, HS group were significantly lower T-AOC levels, significant differences (P<0.01), HS + G mice T-AOC levels increased significantly higher (P < 0.05), G T-AOC mice was significantly increased, with significant differences (P<0.01); and compared HS, HS + G Unit T-AOC was significantly higher difference was significant (P<0.01).3. IL-2:Compared with the C group, HS group IL-2 in spleen lymphocytes were significantly lower difference was significant (P<0.01), HS + G group decreased IL-2 levels, was significantly higher (P<0.05), G group content changes of IL-2 did not change significantly, the difference was not significant (P>0.05). Compared with HS, HS + G group of spleen lymphocytes of IL-2 were significantly higher difference was significant (P<0.01).4. IL-10:Compared with the C group, HS group spleen lymphocytes were significantly increased IL-10, difference was significant (P<0.01), HS + G group IL-10 levels increased, but no significant difference (P>0.05) , G group of lymphocyte IL-10 levels lower, the difference was not significant (P>0.05). Compared with HS, HS + G Group IL-10 levels decreased significantly difference (P<0.01).5. Splenic lymphocyte apoptosis:Compared with the C group, HS group significantly increased the number of cell apoptosis, by analysis, significant differences (P<0.01), HS + G significantly increased the number of apoptotic cells, difference was significant (P<0.01), G reduce the number of spleen lymphocytes, a significant difference (P<0.05). Compared with HS, HS + G mice significantly reduced the number of cells, significant differences (P<0.01)Conclusion:1.EGB high intensity exercise can reduce the serum MDA, reduce free radical damage on cells.2.EGB to some extent high-intensity exercise increased serum activity of SOD, T-AOC, to improve high-intensity exercise by the metabolic disorder caused by free radicals, improve antioxidant capacity.3.EGB as exogenous antioxidants can improve the high-intensity exercise-induced accumulation of free radicals, prevent free radical metabolism, thereby improving the high-intensity exercise the lymphocyte apoptosis, will help improve the immune function .4.EGB to high-intensity exercise in mice splenocytes factor IL-2 increased immunosuppressive factor IL-10 significantly reduced, indicating that high-intensity exercise EGB can improve immune function due to decline in status.