| Background: Multi-country health monitoring system statistics, about 85% of the population 12 to 24 years old during the acne, seriously affecting people's physical and mental health patients. Propionibacterium acnes (P.acnes) in acne formation plays an important role, and its metabolites, such as lipase, protease, hyaluronidase and chemokines can cause inflammation. Skin lipid addition to P.acnes proliferated to provide anaerobic environment, some of which components are directly or indirectly involved in the occurrence of acne inflammation. Photodynamic Therapy is studying the development of new medical technology, the main advantages of photodynamic therapy is to organize selective and will not cause damage to normal tissue. 5-aminolevulinic acid induced protoporphyrin IX has a strong selectivity of the sebaceous glands. Topical ALA in human hair follicles and sebaceous glands become effective transfer photo sens it izer (protoporphyrin IX), transfer into a photos ens itizer in bacteria (fecal or urine porphyrin-porphyrin), caused by the excitation light dynamic response, then inhibition of sebum secretion, inactivation of skin keratinocytes and hair follicles in killing bacteria.Objective: In this study, we aim to investigate the anti-inflammatory effect of photodynamic therapy on Propionibacterium acnes infection induced inflammatory reaction and its possible mechanisms in golden hamster sebaceous patches. For the clinical application of ALA-PDT dermatology treatment acne inflammation and provide experimental evidence. methods: 1 The 16 healthy golden hamsters were randomly divided into 4 groups: I control lgroup, II P.acnes group, III P.acnes + ALA-PDT group, IV P.acnes + red light group. Propionibacterium acnes were cultured and inoculated into II, III, IV golden hamster sebaceous patches to induce inflammatory reaction, 1 week after photodynamic therapy. 2 The group III topical using 20% 5-aminolevulinic acid (ALA) to golden hamster sebaceous patches 1 hour and then red light irradiation, and group IV only red light irradiation. Photodynamic therapy program was once a week for 4 sessions. Irradiation parameters: energy density 127 J/cm2, wavelength 633±3 nm, spot size 1.0 cm, set the time of 15min. ? The sebaceous patche specimens were taken for histopathology examination and special staining. ? Enzyme-linked immunoassay (ELISA) detected serum testosterone and estradiol levels. ? Western blot assay was used to measure protein inflammation signaling pathway expression levels of NF-kB and STAT3.Results:1. Photodynamic therapy could reduce the inflammatory reaction. Compared with P.acnes group, ALA-PDT and red light irradiation alone can reduce the monocytes inflammation, which caused by Propionibacterium acnes. ALA-PDT has the stronger inhibitory effects on the sebaceous gland inflammatory plaque than red light irradiation alone.2. Photodynamic therapy on serum testosterone and estradiol levels. Compared with the control group, ALA-PDT and red light irradiation alone has not change the serum testosterone and estradiol levels , there are no significantly different (P> 0.05).3. Photodynamic therapy on NF-kB and STAT3 protein levels. Compared with P.acnes group, photodynamic therapy decrease NF-kB protein level significantly, while increase expression of STAT3 protein. ALA-PDT group on the sebaceous P.acnes group, photodynamic therapy decrease NF-kB protein level significantly, while increase expression of STAT3 protein. ALA-PDT group on the sebaceous patches inflammation associated protein signaling pathway inhibition has significantly stronger than red light group, the differences are statistically significant (P <0.05).Conclusions: Photodynamic therapy could change the inflammation pathway related protein NF-kB and STAT3 expression. Photodynamic therapy on the sebaceous glands caused by Propionibacterium acnes inflammation has a inhibitory effect. Photodynamic therapy did not affect golden hamster serum testosterone, estradiol expression.
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