The Ecotoxicity Evaluation On The Phthalate Esters

Environmental hormone compounds are a large class of dangerous environmental pollutants presented in the environment, it can change not only the human and wildlife endocrine systems, can lead to abnormal behavior and reproductive development, can affect the quality and plant development growth morphology. Phthalates (Phthalate esters, PAEs) are a class of important synthetic organic compounds, generally used as pesticide carrier, fragrance products, insect repellents, cosmetics, plasticizers, lubricants agents, the production of raw materials to the foam agent and the main purpose plasticizer for plastics. In particular, in recent years as massive production of plastic products and widely being used, phthalate esters continuously into the environment, has become a global one of the most common class of environmental hormonal pollutants.In current studies, four kinds of environmental hormone substances phthalates (DBP, DEP, DOP, and DEHP) are selected as the object of study, from the plant seed enlongation inhibition method to identify inhibition rate of root elongation of the eight plants by the four o-benzene dimethyl ester substances, to understand plants reaction to phthalates in sensitivity. The results show that the most sensitive fibrous roots plant to phthalates can be seen in wheat, phthalates possesses high impact on the relatively large fibrous root plants, while the sensitivitiies of fruit plant to phthalate esters are greater than green vegetable plants. DBP is more toxic to certain types of crops than DEP, DOP and DEHP. Among PAEs, DBP has obvious reproductive toxicity and developmental toxicity. In addition, the micronucleus test of root tip was used to test genetic toxicity of DBP at different concentrations at the cellular level. After processing and determination, wheat and mung bean root tip cells are obviously affected and produced cell micronucleus mitosis. Experimental results show that the cells appeared micronucleus, the greater of the DBP concentration the higher of the rate of micronuclei, chromosome fragments, chromosome and other chromosomal aberrations remain. The micronucleus rate of wheat negative control group was 0.08‰; at the half inhibitory concentration on wheat root elongation (DEP, 80ppm), the micronucleus rate is 3.00‰, about 38 times of the negative control. In wheat high dose group (DEP, 120ppm), the micronucleus rate is 3.58‰, about 45 times of the negative control. At half inhibitory concentration of mung bean root elongation (DEP, 140ppm), the micronucleus rate is 2.80‰, about 35 times of the negative control. In mung bean high dose group (DEP, 200ppm), the micronucleus rate is 2.59‰, about 32 times of the negative control. At the same time, there are many residues of metabolic intermediate, and the metabolism function is decreased. The results indicate that PAEs possess ecotoxcity at individual, physical, cellular and genetic level on the producer of ecosystem.