The Expression And Significance Of Maspin,p27 And B-FGF In Epithelial Ovarian Tumor

Ovarian cancer which is one of the most common malignant tumors of the female reproductive systems holds 2.4%-5.6% of the gynecologic malignancies, and its incidence rate is increased these years. Epithelial ovarian cancer is originated from the germinal epithelium of the ovary. Infiltrating and metastasis are the biocharacteristics of ovarian cancer, and the pathogenesis is not clear. Approximately 70% of patients with ovarian cancer are diagnosed only at advanced disease stages, and the mortality rate of the ovarian cancer is the first of genital organs malignant tumour. There are no specificity and high sensitivity biological markers which can detect the ovarian cancer in the early stage. Therefore, in order to solve the key point of the development of ovarian cancer, we should carry out deepgoing study from genes level, will conduce to the early diagnosis, the early treatment, and the protective measure of the ovarian cancer.Cancer is a proliferative disease characterized by hyperproliferation and insufficient apoptosis. Mammary serpin belongs to serpin superfamily, and participates in many kinds of physiological effects in vivo, include increasing cell adhesion, inhibiting the movement and immigration of tumor cells, resisting the neogenesis of vessel, promoting apoptosis, thus inhibits neoplasm metastasis, and plays an important role in the tumor development.Tumorigeness is closely correlated with the abnormal regulation of cell cycle. P27 is a multifunctional cyclin dependent kinase inhibitor. By preventing the inversion from G0/G1 stage to S stage of cell cycle, p27 can stop the progression of cell cycle. The cyclin-dependent kinase p27 plays an important role in the regulation of cell cycle progression, thereby the negative regulation of cell cycle is accomplished, and then p27 inhibites cell multiplication and tumorigeness.Basic fibroblast growth factor is one of the FGFs, and is widly distributed in varied tissues and organs origined from mesoblastema and neural ectoderm, contains tumors. Basic fibroblast growth factor has a lot of biological effects, such as to promote proliferation, differentiation and metastasize, and participates physiological functions and pathological process, including the remodeling of blood vessels, skeletogeny, neural development and the metabolism of tumor.ObjectiveIn this study, using RT-PCR method we investigated the expression of Maspin mRNA, p27 mRNA and b-FGF mRNA in normal ovary, benign epithelial ovarian tumor and malignant ovarian tumor, and analyzed the relationship of Maspin mRNA, p27 mRNA and b-FGF mRNA, to interpret the correlation between their expression and the biological behaviour of epithelial ovarian cancer, and explore the role of cell growth factor and tumor suppressor gene in the development of ovarian cancer.Methods1 Sampling79 cases accepted operation were selected from the first and the third affiliated hospital of Zhengzhou University from October 2007 and May 2005. The samplings include the normal ovary tissue (n=20),benign epithelial ovarian tumor (n=28) and malignant ovarian tumor (n=31). No any drug was taken preoperatively, also no radiotherapy chemotherapy immunotherapy.2 MethodRT-PCR was used to detect the expression of Maspin mRNA, p27 mRNA and b-FGF mRNA in normal ovary, benign epithelial ovarian tumor and malignant ovarian tumor.3 AssessmentThe gel imaging analytical system was used to scan the PCR production and exam the relative gray value of Maspin, p27 and b-FGF.4 Statistic analysisThe data were recorded and analyzed by using SPSS16.0 package, and were signified by (x±s) for successive type variable, compared in two groups through independent sample t test, three groups through one way analysis of variance. The Pearson product-moment correlation analysis was used to test the data which is normal distribution, and the spearman rank correlation analysis was used to test the nonnormal distribution data.Results1 The expression of Maspin mRNAThe expression of Maspin mRNA was lower in malignant ovarian tumor than the benign epithelial ovarian tumor and the normal ovary. Maspin mRNA were poorly expressed in stageⅢ,Ⅳ, lower than that in stageⅠ,Ⅱ, and they were lower in malignant ovarian tumor with lymphatic metastasis than that negativation.2 The expression of p27 mRNAThe expression of p27 mRNA was lower in malignant ovarian tumor than the benign epithelial ovarian tumor and the normal ovary. p27 mRNA were poorly expressed in stageⅢ,Ⅳ, lower than that in stageⅠ,Ⅱ, and they were lower in malignant ovarian tumor with lymphatic metastasis than that negativation.3 The expression of b-FGF mRNAThe expression of b-FGF mRNA was higher in malignant ovarian tumor than the benign epithelial ovarian tumor and the normal ovary. b-FGF mRNA was strongly expressed in stageⅢ,Ⅳ, higher than that in stageⅠ,Ⅱ, and it was higher in malignant ovarian tumor with lymphatic metastasis than that negativation, and the expression was higher in well differentiated group, compared with poorly differentiated group and moderately differentiated group.4 CorrelationThere was a direct correlation between the expression of Maspin mRNA and p27 mRNA (r=0.688, P<0.001), a negative correlation between the expression of Maspin mRNA and b-FGF mRNA in malignant ovarian tumor (r=-0.418,P=0.019), and there was a negative correlation between the expression of p27 mRNA and b-FGF mRNA (r=-0.434,P=0.015). Conclusion1 The expressions of Maspin mRNA and p27 mRNA in in malignant ovarian tumor were lower, and b-FGF mRNA was higher, which indicated that they might participate in the carcinogenesis and development of ovarian cancer.2 The expressions of Maspin mRNA and p27 mRNA were poorly expressed in stage IV and malignant ovarian tumor with lymphatic metastasis, and their expression were lower with the higher clinical stage, which indicated that their downregulation or lost might participate in the carcinogenesis and development of ovarian tumor. The expressions of b-FGF mRNA were strongly expressed in stage IV and malignant ovarian tumor with lymphatic metastasis, and their expression were higher with the higher clinical stage, which showed that it might be correlation with the degree of malignancy of ovarian cancer.3 There were direct correlations among the expression of Maspin mRNA,p27 mRNA and b-FGF mRNA, which showed that there were connections among them and they promote the the carcinogenesis and development of ovarian cancer together.