Expression And Clinical Significance. Egfr And Downstream Signaling Molecules In Ovarian Cancer

Objective Epidermal growth factor receptor(EGFR) is the heated target spot in ovarian carcinomas in recent years. What's more, it has played positive roles in clinical trails. Furthermore, the research of EGFR's signal transduction pathways and relative signal molecular have attracted more attention. This experiment intended to detect the mRNA levels of EGFR, Akt, Raf-1 in epithelial ovarian carcinomas and investigate their relationships with clinicopathological parameters and their interrelationships.Methods Reverse transcription polymerase chain reaction (RT-PCR) was adopted to determine the expression of EGFR, Akt and Raf-1 mRNA in 14 normal ovarian tumors,16 benign ovarian tumors and 61 malignant epithelial ovarian carcinoma tissues. The relationship between them was analyzed by principle of statistics.Results The positive rates of EGFR, Akt and Raf-1 in patients with epithelial ovarian carcinoma were 78.7%,80.3% and 75.4% respectively, significantly higher than those in ovarian benign tumors and normal controls (P<0.05).There were relations between EGFR, Akt, Raf-1 mRNA expression and FIGO stages, lymphaden metastasis (P<0.05).The expression levels of EGFR and Akt related with cytological grades (P<0.05).The relationships between EGFR and Raf-1, Akt and Raf-1 were significant(P<0.05).Conclusion②The overexpression of EGFR mRNA, Akt mRNA and Raf-1 mRNA were detected in epithelial ovarian carcinomas. There were direct correlation among their expression levels. EGFR mRNA, Akt mRNA and Raf-1 mRNA all positively correlate to the clinical-surgery stages and lymphatic metastasis, which indicated they correlate to the tumorgenesis and development of ovarian carcinomas.②The high expression rates and levels of EGFR, AKT, Raf-1 mRNA in ovarian carcinomas and their close relationship indicated that they may become a new targeted therapy.