| ObjectiveCutaneous malignant melanoma (CMM) is a highly malignant tumor of skin. Its main pathogenesis are cell proliferation and infinite apoptosis. There is no effective prevention and therapy measurement for CMM at present.The researches on the mechanism of CMM showed that CMM is a complex process of multiple injuries in the normal chromosome'. The development, growth and turnover refer to the oncogene activation, tumor suppressor gene inactivation, apoptosis gene disorders, uncontrolled DNA transcription and so on. As a suppressor gene of tumor, its protein can direct the Smad complex from the cytoplasm translate into the nucleus in the TGF-P signal transduction to activate the specific target sites, enhance Smad complex together with the target site of adhesion strength and cell differentiation, cell cycle regulation, apoptosis and malignant transformation of work. Ki-67 is a nuclear antigen that associated with the proliferation. In this research immunohistochemical technique is used to study the expression of Runx3, Smad4, and their relations with the Ki-67 which reflect the proliferation activity, in order to explore Runx3, Smad4 and Ki-67'effection during the generation and development of CMM.Materials and methods38 cases CMM and 20 cases normal skin with complete resection and clear pathological diagnosis were selected which came from the department of pathologyinthe first affiliated hospital of Zhengzhou University. All patients had no merger skin, connective tissue disease, immune disorders and other important organs diseases; Patients were not carried out prior to radiotherapy, chemotherapy, immune, freezing, laser and other treatment.20 cases of normal skin which came from full thick skin grafts were chosen as control groups. The SP immunohistochemical technique was used to detect the expression of Runx3, Smad4 with cell proliferation in the development of CMM. The statistics data were processed by SPSS 13.0, using Chi-square test and Dependability analysis. The test standard is 0.05 and the standard of x2 division is 0.0167.Results1. Runx3 expressed mainly on the nucleus of melanoma cells. There was a statical significance between the CMM and mole skin junction or normal skin(P<0.0167) There is no statistical significance between mole skin junction and normal skin (P> 0.0167)2. Smad4 expressed mainly on the cytoplasm of melanoma cells. There was a statical significance between the CMM and mole skin junction or normal skin (P<0.0167) There was no statistical significance between mole skin junction and normal skin (P >0.0167)3. Ki-67 expressed mainly on the nucleus of melanoma cells. There was a statical significance between the CMM and mole skin junction or normal skin(P<0.0167) There was no statistical significance between mole skin junction and normal skin (P >0.0167)4. The dependability analysis shoewed that there was a significant negative correlation between Runx3 and Smad4 and Ki-67(P<0.05). There was a significant positive correlation between Runx3 and Smad4(r=0.455).Conclusions1. It is speculated that Runx3 and Smad4 might play a tumor suppressor role in CMM for their low expressions. The low expression may promote the formation and development of CMM by promoting cell proliferation and inhibiting cell apoptosis through the cell signaling pathways. 2. The final effect of the TGF-βsignaling system is to promote cell apoptosis and inhibit cell proliferation. As an upstream element, Runx3 can inhibit the development of CMM by guiding the signaling pathway successfully.3. Ki-67 can reflect the proliferation of melanocytes and provide the molecular basis of the degree for the prognosis and development of CMM.
|
PDF Full Text Request